dc.contributor.author
Reuter, Wilhad Hans
dc.contributor.author
Masuch, Thorsten
dc.contributor.author
Ke, Na
dc.contributor.author
Lenon, Marine
dc.contributor.author
Radzinski, Meytal
dc.contributor.author
Loi, Vu Van
dc.contributor.author
Ren, Guoping
dc.contributor.author
Riggs, Paul
dc.contributor.author
Antelmann, Haike
dc.contributor.author
Reichmann, Dana
dc.date.accessioned
2019-12-10T10:40:32Z
dc.date.available
2019-12-10T10:40:32Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26076
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-25835
dc.description.abstract
Understanding the in vivo redox biology of cells is a complex albeit important biological problem. Studying redox processes within living cells without physical disruption or chemical modifications is essential in determining the native redox states of cells. In this study, the previously characterized reduction-oxidation sensitive green fluorescent protein (roGFP2) was used to elucidate the redox changes of the genetically engineered Escherichia coli strain, SHuffle. SHuffle cells were demonstrated to be under constitutive oxidative stress and responding transcriptionally in an OxyR-dependent manner. Using roGFP2 fused to either glutathione (GSH)- or hydrogen peroxide (H2O2)- sensitive proteins (glutaredoxin 1 or Orp1), the cytosolic redox state of both wild type and SHuffle cells based on GSH/GSSG and H2O2 pools was measured. These probes open the path to in vivo studies of redox changes and genetic selections in prokaryotic hosts.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Disulfide bond formation
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
101280
dcterms.bibliographicCitation.doi
10.1016/j.redox.2019.101280
dcterms.bibliographicCitation.journaltitle
Redox Biology
dcterms.bibliographicCitation.volume
26
dcterms.bibliographicCitation.url
https://doi.org/10.1016/j.redox.2019.101280
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Biologie / Arbeitsbereich Mikrobiologie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
2213-2317
dcterms.isPartOf.eissn
2213-2317