dc.contributor.author
Fuhr, Luise
dc.contributor.author
Abreu, Mónica
dc.contributor.author
Carbone, Annalucia
dc.contributor.author
El-Athman, Rukeia
dc.contributor.author
Bianchi, Fabrizio
dc.contributor.author
Laukkanen, Mikko O.
dc.contributor.author
Mazzoccoli, Gianluigi
dc.contributor.author
Relógio, Angela
dc.date.accessioned
2019-12-03T09:38:01Z
dc.date.available
2019-12-03T09:38:01Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26034
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-25793
dc.description.abstract
Cancer cells interrelate with the bordering host microenvironment that encompasses the extracellular matrix and a nontumour cellular component comprising fibroblasts and immune-competent cells. The tumour microenvironment modulates cancer onset and progression, but the molecular factors managing this interaction are not fully understood. Malignant transformation of a benign tumour is among the first crucial events in colorectal carcinogenesis. The role of tumour stroma fibroblasts is well-described in cancer, but less well-characterized in benign tumours. In the current work we utilized fibroblasts isolated from tubulovillous adenoma, which has high risk for malignant transformation, to study the interaction between benign tumour stroma and the circadian clock machinery. We explored the role of the biological clock in this interplay taking advantage of an experimental model, represented by the co-culture of colon cancer cells with normal fibroblasts or tumour-associated fibroblasts, isolated from human colorectal tumour specimens. When co-cultured with tumour-associated fibroblasts, colon cancer cells showed alterations in their circadian and metabolic parameters, with decreased apoptosis, increased colon cancer cell viability, and increased resistance to chemotherapeutic agents. In conclusion, the interactions among colon cancer cells and tumour-associated fibroblasts affect the molecular clockwork and seem to aggravate malignant cell phenotypes, suggesting a detrimental effect of this interplay on cancer dynamics.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
biological clock
en
dc.subject
tumour stroma
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
The Interplay between Colon Cancer Cells and Tumour-Associated Stromal Cells Impacts the Biological Clock and Enhances Malignant Phenotypes
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
E988
dcterms.bibliographicCitation.doi
10.3390/cancers11070988
dcterms.bibliographicCitation.journaltitle
Cancers
dcterms.bibliographicCitation.number
7
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
11
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
31311174
dcterms.isPartOf.eissn
2072-6694