dc.contributor.author
Pfannes, Eva K. B.
dc.contributor.author
Weiss, Lina
dc.contributor.author
Hadam, Sabrina
dc.contributor.author
Gonnet, Jessica
dc.contributor.author
Combardière, Béhazine
dc.contributor.author
Blume-Peytavi, Ulrike
dc.contributor.author
Vogt, Annika
dc.date.accessioned
2019-09-30T10:24:04Z
dc.date.available
2019-09-30T10:24:04Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/25676
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-25440
dc.description.abstract
The success of topically applied treatments on skin relies on the efficacy of skin penetration. In order to increase particle or product penetration, mild skin barrier disruption methods can be used. We previously described cyanoacrylate skin surface stripping as an efficient method to open hair follicles, enhance particle penetration, and activate Langerhans cells. We conducted ex vivo and in vivo measurements on human skin to characterize the biological effect and quantify barrier disruption-related inflammation on a molecular level. Despite the known immunostimulatory effects, this barrier disruption and hair follicle opening method was well accepted and did not result in lasting changes of skin physiological parameters, cytokine production, or clinical side effects. Only in ex vivo human skin did we find a discrete increase in IP-10, TGF-β, IL-8, and GM-CSF mRNA. The data underline the safety profile of this method and demonstrate that the procedure per se does not cause substantial inflammation or skin damage, which is also of interest when applied to non-invasive sampling of biomarkers in clinical trials.
en
dc.subject
Skin barrier
en
dc.subject
Cyanoacrylate skin surface stripping
en
dc.subject
Transcutaneous vaccination
en
dc.subject
Particle penetration
en
dc.subject
Tape stripping
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Physiological and Molecular Effects of in vivo and ex vivo Mild Skin Barrier Disruption
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1159/000484443
dcterms.bibliographicCitation.journaltitle
Skin Pharmacology and Physiology
dcterms.bibliographicCitation.number
3
dcterms.bibliographicCitation.originalpublishername
Karger
dcterms.bibliographicCitation.pagestart
115
dcterms.bibliographicCitation.pageend
124
dcterms.bibliographicCitation.volume
31
dcterms.rightsHolder.note
Copyright applies in this work.
dcterms.rightsHolder.url
http://rightsstatements.org/vocab/InC/1.0/
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.note.author
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
de
refubium.note.author
This publication is shared with permission of the rights owner and made freely accessible through a DFG (German Research Foundation) funded license at either an alliance or national level.
en
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
29510378
dcterms.isPartOf.issn
1660-5527
dcterms.isPartOf.eissn
1660-5535