dc.contributor.author
Wuensch, Tilo
dc.contributor.author
Wizenty, Jonas
dc.contributor.author
Quint, Janina
dc.contributor.author
Spitz, Wolfgang
dc.contributor.author
Bosma, Madeleen
dc.contributor.author
Becker, Olaf
dc.contributor.author
Adler, Andreas
dc.contributor.author
Veltzke-Schlieker, Wilfried
dc.contributor.author
Stockmann, Martin
dc.contributor.author
Weiss, Sascha
dc.contributor.author
Biebl, Matthias
dc.contributor.author
Pratschke, Johann
dc.contributor.author
Aigner, Felix
dc.date.accessioned
2019-08-09T12:36:58Z
dc.date.available
2019-08-09T12:36:58Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/25254
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-3959
dc.description.abstract
Background. Fibronectin type III domain-containing (FNDC) proteins fulfill manifold functions in tissue development and regulation of cellular metabolism. FNDC4 was described as anti-inflammatory factor, upregulated in inflammatory bowel disease (IBD). FNDC signaling includes direct cell-cell interaction as well as release of bioactive peptides, like shown for FNDC4 or FNDC5. The G-protein-coupled receptor 116 (GPR116) was found as a putative FNDC4 receptor. We here aim to comprehensively analyze the mRNA expression of FNDC1, FNDC3A, FNDC3B, FNDC4, FNDC5, and GPR116 in nonaffected and affected mucosal samples of patients with IBD or colorectal cancer (CRC). Methods. Mucosa samples were obtained from 30 patients undergoing diagnostic colonoscopy or from surgical resection of IBD or CRC. Gene expression was determined by quantitative real-time PCR. In addition, FNDC expression data from publicly available Gene Expression Omnibus (GEO) data sets (GDS4296, GDS4515, and GDS5232) were analyzed. Results. Basal mucosal expression revealed higher expression of FNDC3A and FNDC5 in the ileum compared to colonic segments. FNDC1 and FNDC4 were significantly upregulated in IBD. None of the investigated FNDCs was differentially expressed in CRC, just FNDC3A trended to be upregulated. The GEO data set analysis revealed significantly downregulated FNDC4 and upregulated GPR116 in microsatellite unstable (MSI) CRCs. The expression of FNDCs and GPR116 was independent of age and sex. Conclusions. FNDC1 and FNDC4 may play a relevant role in the pathobiology of IBD, but none of the investigated FNDCs is regulated in CRC. GPR116 may be upregulated in advanced or MSI CRC. Further studies should validate the altered FNDC expression results on protein levels and examine the corresponding functional consequences.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Inflammatory Bowel Disease (IBD)
en
dc.subject
Colorectal cancer
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Expression Analysis of Fibronectin Type III Domain-Containing (FNDC) Genes in Inflammatory Bowel Disease and Colorectal Cancer
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
3784172
dcterms.bibliographicCitation.doi
10.1155/2019/3784172
dcterms.bibliographicCitation.journaltitle
Gastroenterology Research and Practice
dcterms.bibliographicCitation.originalpublishername
Hindawi Limited
dcterms.bibliographicCitation.volume
2019
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dc.relation.hascorrection
https://doi.org/10.17169/refubium-28126
dcterms.bibliographicCitation.pmid
31093274
dcterms.isPartOf.issn
1687-6121
dcterms.isPartOf.eissn
1687-630X