dc.contributor.author
Tsvetkov, Dmitry
dc.contributor.author
Kolpakov, Evgeniy
dc.contributor.author
Kassmann, Mario
dc.contributor.author
Schubert, Rudolf
dc.contributor.author
Gollasch, Maik
dc.date.accessioned
2019-07-23T10:17:56Z
dc.date.available
2019-07-23T10:17:56Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/25140
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2895
dc.description.abstract
Perivascular adipose tissue (PVAT) is implicated in the pathophysiology of cardiovascular disease, especially in obese individuals in which the quantity of renal and visceral PVAT is markedly increased. The control of arterial tone by PVAT has emerged as a relatively new field of experimental hypertension research. The discovery of this prototype of vasoregulation has been mostly inferred from data obtained using wire myography. Currently, there is a major discussion on distinguishing between biological vs. technical replicates in biomedical studies, which resulted in numerous guidelines being published on planning studies and publishing data by societies, journals, and associations. Experimental study designs are determined depending on how the experimentator distinguishes between biological vs. technical replicates. These definitions determine the ultimate standards required for making submissions to certain journals. In this article, we examine possible outcomes of different experimental study designs on PVAT control of arterial tone using isolated arteries. Based on experimental data, we determine the sample size and power of statistical analyses for such experiments. We discuss whether n-values should correspond to the number of arterial rings and analyze the resulting effects if those numbers are averaged to provide a single N-value per animal, or whether the hierarchical statistical method represents an alternative for analyzing such kind of data. Our analyses show that that the data (logEC50) from (+) PVAT to (–) PVAT arteries are clustered. Intraclass correlation (ICC) was 31.4%. Moreover, it appeared that the hierarchical approach was better than regular statistical tests as the analyses revealed by a better goodness of fit (v2-2LL test). Based on our results, we propose to use at least three independent arterial rings from each from three animals or at least seven arterial rings from each from two animals for each group, i.e., (+) PVAT vs. (–) PVAT. Finally, we discuss a clinical situation where distinguishing between biological vs. technical replicates can lead to absurd situations in clinical decision makings. We conclude that discrimination between biological vs. technical replicates is helpful in experimental studies but is difficult to implement in everyday’s clinical practice.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
adipocyte-derived relaxing factor (ADRF)
en
dc.subject
perivascular adipose tissue (PVAT)
en
dc.subject
biological replicates
en
dc.subject
experimental study design
en
dc.subject
power of statistical analysis
en
dc.subject
hierarchical model
en
dc.subject
technical replicates
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Distinguishing Between Biological and Technical Replicates in Hypertension Research on Isolated Arteries
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
126
dcterms.bibliographicCitation.doi
10.3389/fmed.2019.00126
dcterms.bibliographicCitation.journaltitle
Frontiers in Medicine
dcterms.bibliographicCitation.originalpublishername
Frontiers Media S.A.
dcterms.bibliographicCitation.volume
6
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
31281816
dcterms.isPartOf.eissn
2296-858X