dc.contributor.author
Veiga, Inês M. B.
dc.contributor.author
Lüschow, Dörte
dc.contributor.author
Gutzer, Stefanie
dc.contributor.author
Hafez, Hafez M.
dc.contributor.author
Mühldorfer, Kristin
dc.date.accessioned
2019-07-18T07:26:33Z
dc.date.available
2019-07-18T07:26:33Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/25104
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2859
dc.description.abstract
Background
Ornithobacterium (O.) rhinotracheale is an emerging bacterial pathogen in poultry and not fully understood to date. Because of its importance particularly for the global turkey meat industry, reliable diagnostic and characterization methods are needed for early treatment and in future for better vaccine production. The host range of birds infected by O. rhinotracheale or carrying the bacterium in their respiratory tract has constantly increased raising important epidemiological and taxonomic questions for a better understanding of its diversity, ecology and transmission cycles. The purpose of this study was to introduce partial rpoB gene sequencing for O. rhinotracheale into routine diagnostics to differentiate strains isolated from poultry and more diverse avian hosts (i.e., birds of prey, corvids and pigeons) and to compare phylogenetic relationships with results from 16S rRNA gene analysis and multilocus sequence typing (MLST).
Results
Partial 16S rRNA gene analysis revealed a high level of homogeneity among the 65 investigated O. rhinotracheale sequences with similarity values ranging from 98.6 to 100% between sequences from non-galliform and poultry species. The corresponding rpoB gene sequences were heterogeneous and ranged in their similarity values from 85.1 to 100%. The structure of the rpoB tree was in strong correlation with previous MLST results revealing three main clusters A (poultry and birds of prey), B (poultry, birds of prey and corvids) and C (pigeons), which were clearly separated from each other.
Conclusions
By using partial sequences from a single gene, the rpoB gene analysis is in good agreement with MLST results with a slight decrease in resolution to distinguish more similar strains. The present results provide strong evidence that traditional phenotypic and genetic methods may not properly represent the heterogeneous group of bacteria classified as O. rhinotracheale. From housekeeping gene analyses, it is very likely that the genus Ornithobacterium includes additional species and partial rpoB gene sequencing can be recommended as fast, cost-effective and readily available method to identify strains and differentiate between O. rhinotracheale and Ornithobacterium-like bacteria.
en
dc.format.extent
7 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
flavobacteriaceae
en
dc.subject
ornithobacterium
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::598 Aves (Vögel)
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Phylogenetic relationship of Ornithobacterium rhinotracheale isolated from poultry and diverse avian hosts based on 16S rRNA and rpoB gene analyses
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
31
dcterms.bibliographicCitation.doi
10.1186/s12866-019-1395-9
dcterms.bibliographicCitation.journaltitle
BMC Microbiology
dcterms.bibliographicCitation.volume
19
dcterms.bibliographicCitation.url
https://doi.org/10.1186/s12866-019-1395-9
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Virologie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1471-2180
refubium.resourceType.provider
WoS-Alert