dc.contributor.author
Ahmed, Norus
dc.contributor.author
Heitlinger, Emanuel
dc.contributor.author
Affinass, Nicole
dc.contributor.author
Kühl, Anja A.
dc.contributor.author
Xenophontos, Natasa
dc.contributor.author
Jarquin, Victor Hugo
dc.contributor.author
Jost, Jenny
dc.contributor.author
Steinfelder, Svenja
dc.contributor.author
Hartmann, Susanne
dc.date.accessioned
2019-07-15T10:58:31Z
dc.date.available
2019-07-15T10:58:31Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/25064
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2819
dc.description.abstract
Currently, methods for monitoring changes of gut barrier integrity and the associated immune response via non-invasive means are limited. Therefore, we aimed to develop a novel non-invasive technique to investigate immunological host responses representing gut barrier changes in response to infection. We identified the mucous layer on feces from mice to be mainly composed of exfoliated intestinal epithelial cells. Expression of RELM-β, a gene prominently expressed in intestinal nematode infections, was used as an indicator of intestinal cellular barrier changes to infection. RELM-β was detected as early as 6 days post-infection (dpi) in exfoliated epithelial cells. Interestingly, RELM-β expression also mirrored the quality of the immune response, with higher amounts being detectable in a secondary infection and in high dose nematode infection in laboratory mice. This technique was also applicable to captured worm-infected wild house mice. We have therefore developed a novel non-invasive method reflecting gut barrier changes associated with alterations in cellular responses to a gastrointestinal nematode infection.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
intestinal nematode
en
dc.subject
non-invasive method
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::632 Schäden, Krankheiten, Schädlinge an Pflanzen
dc.title
A Novel Non-invasive Method to Detect RELM Beta Transcript in Gut Barrier Related Changes During a Gastrointestinal Nematode Infection
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
445
dcterms.bibliographicCitation.doi
10.3389/fimmu.2019.00445
dcterms.bibliographicCitation.journaltitle
Frontiers in Immunology
dcterms.bibliographicCitation.volume
10
dcterms.bibliographicCitation.url
https://doi.org/10.3389/fimmu.2019.00445
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Immunologie
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin und der DFG gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1664-3224