dc.contributor.author
Soltan, Marwa Y.
dc.contributor.author
Sumarni, Uly
dc.contributor.author
Assaf, Chalid
dc.contributor.author
Langer, Peter
dc.contributor.author
Reidel, Ulrich
dc.contributor.author
Eberle, Jürgen
dc.date.accessioned
2019-06-11T09:29:37Z
dc.date.available
2019-06-11T09:29:37Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/24723
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2483
dc.description.abstract
Cutaneous T-cell lymphoma (CTCL) may develop a highly malignant phenotype in its late phase, and patients may profit from innovative therapies. The plant extract indirubin and its chemical derivatives represent new and promising antitumor strategies. This first report on the effects of an indirubin derivative in CTCL cells shows a strong decrease of cell proliferation and cell viability as well as an induction of apoptosis, suggesting indirubin derivatives for therapy of CTCL. As concerning the mode of activity, the indirubin derivative DKP-071 activated the extrinsic apoptosis cascade via caspase-8 and caspase-3 through downregulation of the caspase antagonistic proteins c-FLIP and XIAP. Importantly, a strong increase of reactive oxygen species (ROS) was observed as an immediate early effect in response to DKP-071 treatment. The use of antioxidative pre-treatment proved the decisive role of ROS, which turned out upstream of all other proapoptotic effects monitored. Thus, reactive oxygen species appear as a highly active proapoptotic pathway in CTCL, which may be promising for therapeutic intervention. This pathway can be efficiently activated by an indirubin derivative.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
cell viability
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Key Role of Reactive Oxygen Species (ROS) in Indirubin Derivative-Induced Cell Death in Cutaneous T-Cell Lymphoma Cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1158
dcterms.bibliographicCitation.doi
10.3390/ijms20051158
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
5
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
20
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
30866411
dcterms.isPartOf.issn
1422-0067