dc.contributor.author
Stich, Stefan
dc.contributor.author
Möller, Anke
dc.contributor.author
Cabraja, Mario
dc.contributor.author
Krüger, Jan Philipp
dc.contributor.author
Hondke, Sylvia
dc.contributor.author
Endres, Michaela
dc.contributor.author
Ringe, Jochen
dc.contributor.author
Sittinger, Michael
dc.date.accessioned
2019-04-10T12:48:17Z
dc.date.available
2019-04-10T12:48:17Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/24358
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2130
dc.description.abstract
Intervertebral disc degeneration is a major source of back pain. For intervertebral disc regeneration after herniation a fast closure of anulus fibrosus (AF) defects is crucial. Here, the use of the C-C motif chemokine ligand 25 (CCL)25 in comparison to differentiation factors such as transforming growth factor (TGF)beta 3, bone morphogenetic protein (BMP)2, BMP7, BMP12, and BMP14 (all in concentrations of 10, 50 and 100 ng/mL) was tested in an in vitro micro mass pellet model with isolated and cultivated human AF-cells (n = 3) to induce and enhance AF-matrix formation. The pellets were differentiated (serum-free) with supplementation of the factors. After 28 days all used factors induced proteoglycan production (safranin O staining) and collagen type I production (immunohistochemical staining) in at least one of the tested concentrations. Histomorphometric scoring revealed that TGF beta 3 delivered the strongest induction of proteoglycan production in all three concentrations. Furthermore, it was the only factor able to facilitate collagen type II production, even higher than in native tissue samples. CCL25 was also able to induce proteoglycan and collagen type I production comparable to several BMPs. CCL25 could additionally induce migration of AF-cells in a chemotaxis assay and therefore possibly aid in regeneration processes after disc herniation by recruiting AF-cells.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
anulus fibrosus
en
dc.subject
differentiation
en
dc.subject
extracellular matrix
en
dc.subject
tissue regeneration
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Chemokine CCL25 induces migration and extracellular matrix production of anulus fibrosus-derived cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2207
dcterms.bibliographicCitation.doi
10.3390/ijms19082207
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
8
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
19
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
30060561
dcterms.isPartOf.issn
1422-0067