dc.contributor.author
Muth, Doreen
dc.contributor.author
Corman, Victor Max
dc.contributor.author
Roth, Hanna
dc.contributor.author
Binger, Tabea
dc.contributor.author
Dijkman, Ronald
dc.contributor.author
Gottula, Lina Theresa
dc.contributor.author
Gloza-Rausch, Florian
dc.contributor.author
Balboni, Andrea
dc.contributor.author
Battilani, Mara
dc.contributor.author
Rihtarič, Danijela
dc.contributor.author
Toplak, Ivan
dc.contributor.author
Ameneiros, Ramon Seage
dc.contributor.author
Pfeifer, Alexander
dc.contributor.author
Thiel, Volker
dc.contributor.author
Drexler, Jan Felix
dc.contributor.author
Mueller, Marcel Alexander
dc.contributor.author
Drosten, Christian
dc.date.accessioned
2019-04-03T09:44:59Z
dc.date.available
2019-04-03T09:44:59Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/24284
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2056
dc.description.abstract
A 29 nucleotide deletion in open reading frame 8 (ORF8) is the most obvious genetic change in severe acute respiratory syndrome coronavirus (SARS-CoV) during its emergence in humans. In spite of intense study, it remains unclear whether the deletion actually reflects adaptation to humans. Here we engineered full, partially deleted (-29 nt), and fully deleted ORF8 into a SARS-CoV infectious cDNA clone, strain Frankfurt-1. Replication of the resulting viruses was compared in primate cell cultures as well as Rhinolophus bat cells made permissive for SARS-CoV replication by lentiviral transduction of the human angiotensin-converting enzyme 2 receptor. Cells from cotton rat, goat, and sheep provided control scenarios that represent host systems in which SARS-CoV is neither endemic nor epidemic. Independent of the cell system, the truncation of ORF8 (29 nt deletion) decreased replication up to 23-fold. The effect was independent of the type I interferon response. The 29 nt deletion in SARS-CoV is a deleterious mutation acquired along the initial human-to-human transmission chain. The resulting loss of fitness may be due to a founder effect, which has rarely been documented in processes of viral emergence. These results have important implications for the retrospective assessment of the threat posed by SARS.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
SARS-coronavirus
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
15177
dcterms.bibliographicCitation.doi
10.1038/s41598-018-33487-8
dcterms.bibliographicCitation.journaltitle
Scientific Reports
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
Nature Publishing Group
dcterms.bibliographicCitation.volume
8
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
30310104
dcterms.isPartOf.eissn
2045-2322