dc.contributor.author
Mertens, Robert
dc.contributor.author
Melchert, Sarah
dc.contributor.author
Gitler, Daniel
dc.contributor.author
Schou, Morten Brix
dc.contributor.author
Saether, Sverre Georg
dc.contributor.author
Vaaler, Arne
dc.contributor.author
Piepgras, Johannes
dc.contributor.author
Kochova, Elena
dc.contributor.author
Benfenati, Fabio
dc.contributor.author
Ahnert-Hilger, Gudrun
dc.contributor.author
Ruprecht, Klemens
dc.contributor.author
Höltje, Markus
dc.date.accessioned
2019-03-29T10:07:10Z
dc.date.available
2019-03-29T10:07:10Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/24227
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-1999
dc.description.abstract
Objective: To identify the specific domains of the presynaptic protein synapsin targeted by recently described autoantibodies to synapsin. Methods: Sera of 20 and CSF of two patients with different psychiatric and neurological disorders previously tested positive for immunoglobulin (Ig)G antibodies to full-length synapsin were screened for IgG against synapsin I domains using HEK293 cells transfected with constructs encoding different domains of rat synapsin Ia. Additionally, IgG subclasses were determined using full-length synapsin Ia. Serum and CSF from one patient were also screened for IgA autoantibodies to synapsin I domains. Sera from nine and CSF from two healthy subjects were analyzed as controls. Results: IgG in serum from 12 of 20 IgG synapsin full-length positive patients, but from none of the healthy controls, bound to synapsin domains. Of these 12 sera, six bound to the A domain, five to the D domain, and one to the B- (and possibly A-), D-, and E-domains of synapsin I. IgG antibodies to the D-domain were also detected in one of the CSF samples. Determination of IgG subclasses detected IgG(1) in two sera and one CSF, IgG(2) in none of the samples, IgG(3) in two sera, and IgG(4) in eight sera. One patient known to be positive for IgA antibodies to full-length synapsin had IgA antibodies to the D-domain in serum and CSF. Conclusions: Anti-synapsin autoantibodies preferentially bind to either the A- or the D-domain of synapsin I.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Epitope specificity of anti-synapsin autoantibodies: Differential targeting of synapsin I domains
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e0208636
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0208636
dcterms.bibliographicCitation.journaltitle
PLoS ONE
dcterms.bibliographicCitation.number
12
dcterms.bibliographicCitation.originalpublishername
Public Library of Science (PLoS)
dcterms.bibliographicCitation.volume
13
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
30543686
dcterms.isPartOf.issn
1932-6203