dc.contributor.author
Oliveira, Victor F.
dc.contributor.author
Moares, Lauro A. G.
dc.contributor.author
Mota, Ester A.
dc.contributor.author
Jannotti-Passos, Liana K.
dc.contributor.author
Coelho, Paulo M. Z.
dc.contributor.author
Mattos, Ana C. A.
dc.contributor.author
Couto, Flávia F. B.
dc.contributor.author
Caffrey, Brian E.
dc.contributor.author
Marsico, Annalisa
dc.contributor.author
Guerra-Sá, Renata
dc.date.accessioned
2018-09-26T12:53:12Z
dc.date.available
2018-09-26T12:53:12Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/23009
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-807
dc.description.abstract
Long noncoding RNAs (lncRNAs) are transcripts generally longer than 200 nucleotides with no or poor protein coding potential, and most of their functions are also poorly characterized. Recently, an increasing number of studies have shown that lncRNAs can be involved in various critical biological processes such as organism development or cancer progression. Little, however, is known about their effects in helminths parasites, such as Schistosoma mansoni. Here, we present a computational pipeline to identify and characterize lncRNAs from RNA-seq data with high confidence from S. mansoni adult worms. Through the utilization of different criteria such as genome localization, exon number, gene length, and stability, we identified 170 new putative lncRNAs. All novel S. mansoni lncRNAs have no conserved synteny including human and mouse. These closest protein coding genes were enriched in 10 significant Gene Ontology terms related to metabolism, transport, and biosynthesis. Fifteen putative lncRNAs showed differential expression, and three displayed sex-specific differential expressions in praziquantel sensitive and resistant adult worm couples. Together, our method can predict a set of novel lncRNAs from the RNA-seq data. Some lncRNAs are shown to be differentially expressed suggesting that those novel lncRNAs can be given high priority in further functional studies focused on praziquantel resistance.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
de
dc.subject
Schistosoma mansoni
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::500 Naturwissenschaften::500 Naturwissenschaften und Mathematik
de
dc.title
Identification of 170 New Long Noncoding RNAs in Schistosoma mansoni
de
dc.type
Wissenschaftlicher Artikel
de
dcterms.bibliographicCitation.articlenumber
1264697
dcterms.bibliographicCitation.doi
10.1155/2018/1264697
dcterms.bibliographicCitation.journaltitle
BioMed Research International
dcterms.bibliographicCitation.volume
2018
dcterms.bibliographicCitation.url
https://doi.org/10.1155/2018/1264697
de
refubium.affiliation
Mathematik und Informatik
de
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
de
refubium.resourceType.isindependentpub
no
de
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
2314-6133 (Print)
dcterms.isPartOf.issn
2314-6141 (Online)