dc.contributor.author
Nash, Maisie V.
dc.contributor.author
Anesio, Alexandre M.
dc.contributor.author
Barker, Gary
dc.contributor.author
Tranter, Martyn
dc.contributor.author
Varliero, Gilda
dc.contributor.author
Eloe-Fadrosh, Emiley A.
dc.contributor.author
Nielsen, Torben
dc.contributor.author
Turpin-Jelfs, Thomas
dc.contributor.author
Benning, Liane G.
dc.contributor.author
S´anchez-Baracaldo, Patricia
dc.date.accessioned
2018-09-12T10:14:50Z
dc.date.available
2018-09-12T10:14:50Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/22837
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-636
dc.description.abstract
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes.
en
dc.format.extent
12 S.
de_DE
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
de_DE
dc.subject
nitrogen fixation
en
dc.subject
metagenomics
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen, Pilze, Algen
de_DE
dc.title
Metagenomic insights into diazotrophic communities across Arctic glacier forefields
de_DE
dc.type
Wissenschaftlicher Artikel
de_DE
dcterms.bibliographicCitation.articlenumber
fiy114
dcterms.bibliographicCitation.doi
10.1093/femsec/fiy114
dcterms.bibliographicCitation.journaltitle
FEMS Microbiology Ecology
dcterms.bibliographicCitation.number
9
dcterms.bibliographicCitation.volume
94
dcterms.bibliographicCitation.url
https://doi.org/10.1093/femsec/fiy114
de_DE
refubium.affiliation
Biologie, Chemie, Pharmazie
de_DE
refubium.resourceType.isindependentpub
no
de_DE
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1574-6941 (Online)
dcterms.isPartOf.issn
0168-6496 (Print)