dc.contributor.author
Aguilera-Rojas, Matias Ignacio
dc.contributor.author
Badewien-Rentzsch, Brit
dc.contributor.author
Plendl, Johanna
dc.contributor.author
Kohn, Barbara
dc.contributor.author
Einspanier, Ralf
dc.date.accessioned
2018-08-02T10:14:39Z
dc.date.available
2018-08-02T10:14:39Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/22603
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-409
dc.description.abstract
Exosomes are defined as extracellular membrane vesicles, 30–150 nm in diameter, derived from all types of cells. They originate via endocytosis and then they are released through exocytosis to the extracellular space, being found in various biological fluids as well as in cell culture medium. In the last few years, exosomes have gained considerable scientific interest due to their potential use as biomarkers, especially in the field of cancer research. This report describes a method to isolate, quantify and identify serum- and cell culture-derived exosomes from dog samples, using small volumes (100 μL and 1 mL, respectively).
en
dc.format.extent
9 Seiten
de
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
de
dc.subject
Cell culture medium
en
dc.subject
Transmission electron microscopy
en
dc.subject
Nanoparticle tracking analysis
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::599 Mammalia (Säugetiere)
de
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
de
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::632 Schäden, Krankheiten, Schädlinge an Pflanzen
de
dc.title
Exploration of serum- and cell culture-derived exosomes from dogs
de
dc.type
Wissenschaftlicher Artikel
de
dcterms.bibliographicCitation.articlenumber
179
dcterms.bibliographicCitation.doi
10.1186/s12917-018-1509-x
dcterms.bibliographicCitation.journaltitle
BMC Veterinary Research
dcterms.bibliographicCitation.volume
14
dcterms.bibliographicCitation.url
https://doi.org/10.1186/s12917-018-1509-x
de
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Veterinär-Biochemie
de
refubium.funding
Deutsche Forschungsgemeinschaft (DFG)
de
refubium.note.author
Gefördert durch die DFG und den Open-Access-Publikationsfonds der Freien Universität Berlin.
de
refubium.resourceType.isindependentpub
no
de
dcterms.accessRights.dnb
free
de
dcterms.accessRights.openaire
open access
