dc.contributor.author
Engelmann, Cornelius
dc.contributor.author
Splith, Katrin
dc.contributor.author
Krohn, Sandra
dc.contributor.author
Herber, Adam
dc.contributor.author
Boehlig, Albrecht
dc.contributor.author
Boehm, Stephan
dc.contributor.author
Pratschke, Johann
dc.contributor.author
Berg, Thomas
dc.contributor.author
Schmelzle, Moritz
dc.date.accessioned
2018-06-08T11:07:49Z
dc.date.available
2017-10-13T12:39:01.042Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21672
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24960
dc.description.abstract
Background Microparticles (MPs) are small (<1 μm) cell membrane-derived
vesicles that are formed in response to cellular activation or early stages of
apoptosis. Increased plasma MP levels have been associated with liver disease
severity. Here we investigated the clinical impact of ascites MPs in patients
with decompensated liver cirrhosis. Methods Ascites and blood samples of 163
patients with cirrhosis (ascites n = 163, blood n = 31) were collected between
February 2011 and December 2012. MPs were obtained from ascites and from blood
by two-step ultracentrifugation and quantified by flow cytometry. Quantitative
absolute MP levels were correlated with clinical and laboratory baseline
parameters as well as patient outcomes. Ascites microparticles were stained
with antibodies against CD66b (neutrophils) and CD3 (lymphocytes) in a
subgroup of 60 matched patients. Results MPs were detected in all ascites and
blood samples. Absolute ascites MP levels correlated with blood levels (r =
0.444, p = 0.011). Low ascites MP levels (<488.4 MP/μL) were associated with a
poor 30-day survival probability (<488.4 MP/μL 71.1% vs. >488.4 MP/μL 94.7%,
log rank p = 0.001) and such patients had a higher relative amount of ascites
microparticles derived from neutrophils and lymphocytes. Low levels of ascites
MPs, high MELD score and antibiotic treatment were independent risk factors
for death within 30 days. Conclusions Ascites MP levels predict short-term
survival along with the liver function in patients with decompensated
cirrhosis. Further studies which evaluate ascites MPs as disease specific
biomarker with a validation cohort and which investigate its underlying
mechanisms are needed. Neutrophils and lymphocytes contributed more frequently
to the release of microparticles in patients with low ascites levels, possibly
indicating an immune activation in this cohort.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Microparticles
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Absolute quantification of microparticles by flow cytometry in ascites of
patients with decompensated cirrhosis
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Journal of Translational Medicine. - 188 (2017), Artikel Nr. 188
dc.title.subtitle
a cohort study
dcterms.bibliographicCitation.doi
10.1186/s12967-017-1288-3
dcterms.bibliographicCitation.url
http://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1288-3
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000028310
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000008986
dcterms.accessRights.openaire
open access