dc.contributor.author
Ranjan, Amit
dc.contributor.author
Shaik, Sabiha
dc.contributor.author
Nandanwar, Nishant
dc.contributor.author
Hussain, Arif
dc.contributor.author
Tiwari, Sumeet K.
dc.contributor.author
Semmler, Torsten
dc.contributor.author
Jadhav, Savita
dc.date.accessioned
2018-06-08T11:07:33Z
dc.date.available
2017-10-17T12:18:29.757Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21663
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24951
dc.description.abstract
Escherichia coli, an intestinal Gram-negative bacterium, has been shown to be
associated with a variety of diseases in addition to intestinal infections,
such as urinary tract infections (UTIs), meningitis in neonates, septicemia,
skin and soft tissue infections (SSTIs), and colisepticemia. Thus, for
nonintestinal infections, it is categorized as extraintestinal pathogenic E.
coli (ExPEC). It is also an opportunistic pathogen, causing cross infections,
notably as an agent of zoonotic diseases. However, comparative genomic data
providing functional and genetic coordinates for ExPEC strains associated with
these different types of infections have not proven conclusive. In the study
reported here, ExPEC E. coli isolated from SSTIs was characterized, including
virulence and drug resistance profiles, and compared with isolates from
patients suffering either pyelonephritis or septicemia. Results revealed that
the majority of the isolates belonged to two pathogenic phylogroups, B2 and D.
Approximately 67% of the isolates were multidrug resistant (MDR), with 85%
producing extended-spectrum beta-lactamase (ESBL) and 6% producing metallo-
beta-lactamase (MBL). The blaCTX-M-15 genotype was observed in at least 70% of
the E. coli isolates in each category, conferring resistance to an extended
range of beta-lactam antibiotics. Whole-genome sequencing and comparative
genomics of the ExPEC isolates revealed that two of the four isolates from
SSTIs, NA633 and NA643, belong to pandemic sequence type ST131, whereas
functional characteristics of three of the ExPEC pathotypes revealed that they
had equal capabilities to form biofilm and were resistant to human serum.
Overall, the isolates from a variety of ExPEC infections demonstrated similar
resistomes and virulomes and did not display any disease-specific functional
or genetic coordinates. IMPORTANCE Infections caused by extraintestinal
pathogenic E. coli (ExPEC) are of global concern as they result in significant
costs to health care facilities management. The recent emergence of a
multidrug-resistant pandemic clone, Escherichia coli ST131, is of primary
concern as a global threat. In developing countries, such as India, skin and
soft tissue infections (SSTIs) associated with E. coli are marginally
addressed. In this study, we employed both genomic analysis and phenotypic
assays to determine relationships, if any, among the ExPEC pathotypes.
Similarity between antibiotic resistance and virulence profiles was observed,
ST131 isolates from SSTIs were reported, and genomic similarities among
strains isolated from different disease conditions were detected. This study
provides functional molecular infection epidemiology insight into SSTI-
associated E. coli compared with ExPEC pathotypes.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen, Pilze, Algen
dc.title
Comparative Genomics of Escherichia coli Isolated from Skin and Soft Tissue
and Other Extraintestinal Infections
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
mBio. - 8 (2017), 4, Artikel Nr. e01070-17
dcterms.bibliographicCitation.doi
10.1128/mBio.01070-17
dcterms.bibliographicCitation.url
http://mbio.asm.org/content/8/4/e01070-17
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000028329
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000008999
dcterms.accessRights.openaire
open access