dc.contributor.author
Kenny, Elaine F.
dc.contributor.author
Herzig, Alf
dc.contributor.author
Krüger, Renate
dc.contributor.author
Muthu, Aaron
dc.contributor.author
Mondal, Santanu
dc.contributor.author
Thompson, Paul R.
dc.contributor.author
Brinkman, Volker
dc.contributor.author
von Bernuth, Horst
dc.contributor.author
Zychlinsky, Arturo
dc.date.accessioned
2018-06-08T11:03:31Z
dc.date.available
2017-09-04T09:43:28.771Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21547
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24837
dc.description.abstract
Neutrophils release neutrophil extracellular traps (NETs) which ensnare
pathogens and have pathogenic functions in diverse diseases. We examined the
NETosis pathways induced by five stimuli; PMA, the calcium ionophore A23187,
nigericin, Candida albicans and Group B Streptococcus. We studied NET
production in neutrophils from healthy donors with inhibitors of molecules
crucial to PMA-induced NETs including protein kinase C, calcium, reactive
oxygen species, the enzymes myeloperoxidase (MPO) and neutrophil elastase.
Additionally, neutrophils from chronic granulomatous disease patients,
carrying mutations in the NADPH oxidase complex or a MPO-deficient patient
were examined. We show that PMA, C. albicans and GBS use a related pathway for
NET induction, whereas ionophores require an alternative pathway but that NETs
produced by all stimuli are proteolytically active, kill bacteria and composed
mainly of chromosomal DNA. Thus, we demonstrate that NETosis occurs through
several signalling mechanisms, suggesting that extrusion of NETs is important
in host defence.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Diverse stimuli engage different neutrophil extracellular trap pathways
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
eLife. - 6 (2017), Artikel Nr. e24437
dcterms.bibliographicCitation.doi
10.7554/eLife.24437
dcterms.bibliographicCitation.url
http://doi.org/10.7554/eLife.24437.001
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000027810
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000008693
dcterms.accessRights.openaire
open access