dc.contributor.author
Broggini, Thomas
dc.contributor.author
Piffko, Andras
dc.contributor.author
Hoffmann, Christian J.
dc.contributor.author
Harms, Christoph
dc.contributor.author
Vajkoczy, Peter
dc.contributor.author
Czabanka, Marcus
dc.date.accessioned
2018-06-08T11:00:19Z
dc.date.available
2016-11-03T12:35:21.654Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21455
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24748
dc.description.abstract
During the metastatic process tumor cells circulate in the blood stream and
are carried to various organs. In order to spread to different organs tumor
cell—endothelial cell interactions are crucial for extravasation mechanisms.
It remains unclear if tumor cell dissemination to the spinal bone occurs by
passive entrapment of circulating tumor cells or by active cellular mechanisms
mediated by cell surface molecules or secreted factors. We investigated the
seeding of three different tumor cell lines (melanoma, lung and prostate
carcinoma) to the microvasculature of different organs. Their dissemination
was compared to biologically passive microbeads. The spine and other organs
were resected three hours after intraarterial injection of tumor cells or
microbeads. Ex vivo homogenization and fluorescence analysis allowed
quantification of tumor cells or microbeads in different organs.
Interestingly, tumor cell distribution to the spinal bone was comparable to
dissemination of microbeads independent of the tumor cell type (melanoma:
5.646% ± 7.614%, lung: 6.007% ± 1.785%, prostate: 3.469% ± 0.602%, 7 μm beads:
9.884% ± 7.379%, 16 μm beads: 7.23% ± 1.488%). Tumor cell seeding differed
significantly between tumor cells and microbeads in all soft tissue organs.
Moreover, there were significant differences between the different tumor cell
lines in their dissemination behaviour to soft tissue organs only. These
findings demonstrate that metastatic dissemination of tumor cells to spinal
bone and other osseous organs is mediated by passive entrapment of tumor cells
similar to passive plugging of microvasculature observed after intraarterial
microbeads injection.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Passive Entrapment of Tumor Cells Determines Metastatic Dissemination to
Spinal Bone and Other Osseous Tissues
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 11 (2016), 9, Artikel Nr. e0162540
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0162540
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1371/journal.pone.0162540
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000025659
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007304
dcterms.accessRights.openaire
open access