dc.contributor.author
Platteel, Anouk C. M.
dc.contributor.author
Liepe, Juliane
dc.contributor.author
Textoris-Taube, Kathrin
dc.contributor.author
Keller, Christin
dc.contributor.author
Henklein, Petra
dc.contributor.author
Schalkwijk, Hanna H.
dc.contributor.author
Cardoso, Rebeca
dc.contributor.author
Kloetzel, Peter M.
dc.contributor.author
Mishto, Michele
dc.contributor.author
Sijts, Alice J. A. M.
dc.date.accessioned
2018-06-08T10:59:28Z
dc.date.available
2017-09-08T09:57:59.016Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21441
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24734
dc.description.abstract
Proteasome-catalyzed peptide splicing (PCPS) generates peptides that are
presented by MHC class I molecules, but because their identification is
challenging, the immunological relevance of spliced peptides remains unclear.
Here, we developed a reverse immunology-based multi-level approach to identify
proteasome-generated spliced epitopes. Applying this strategy to a murine
Listeria monocytogenes infection model, we identified two spliced epitopes
within the secreted bacterial phospholipase PlcB that primed antigen-specific
CD8+ T cells in L. monocytogenes-infected mice. While reacting to the spliced
epitopes, these CD8+ T cells failed to recognize the non-spliced peptide parts
in the context of their natural flanking sequences. Thus, we here show that
PCPS expands the CD8+ T cell response against L. monocytogenes by exposing
spliced epitopes on the cell surface. Moreover, our multi-level strategy opens
up opportunities to systematically investigate proteins for spliced epitope
candidates and thus strategies for immunotherapies or vaccine design.
de
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes
Targeted by CD8+ T Cells during Bacterial Infection
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Cell Reports. - 20 (2017), 5, S. 1242–1253
dcterms.bibliographicCitation.doi
10.1016/j.celrep.2017.07.026
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1016/j.celrep.2017.07.026
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000027897
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000008719
dcterms.accessRights.openaire
open access