dc.contributor.author
Terry-Lorenzo, Ryan T.
dc.contributor.author
Torres, Viviana I.
dc.contributor.author
Wagh, Dhananjay
dc.contributor.author
Galaz, Jose
dc.contributor.author
Swanson, Selene K.
dc.contributor.author
Florens, Laurence
dc.contributor.author
Washburn, Michael P.
dc.contributor.author
Waites, Clarissa L.
dc.contributor.author
Gundelfinger, Eckart D.
dc.contributor.author
Reimer, Richard J.
dc.contributor.author
Garner, Craig C.
dc.date.accessioned
2018-06-08T10:55:56Z
dc.date.available
2017-02-02T13:09:12.859Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21354
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24648
dc.description.abstract
Synaptic vesicles (SVs) fuse with the plasma membrane at a precise location
called the presynaptic active zone (AZ). This fusion is coordinated by
proteins embedded within a cytoskeletal matrix assembled at the AZ (CAZ). In
the present study, we have identified a novel binding partner for the CAZ
proteins Piccolo and Bassoon. This interacting protein, Trio, is a member of
the Dbl family of guanine nucleotide exchange factors (GEFs) known to regulate
the dynamic assembly of actin and growth factor dependent axon guidance and
synaptic growth. Trio was found to interact with the C-terminal PBH 9/10
domains of Piccolo and Bassoon via its own N-terminal Spectrin repeats, a
domain that is also critical for its localization to the CAZ. Moreover, our
data suggest that regions within the C-terminus of Trio negatively regulate
its interactions with Piccolo/Bassoon. These findings provide a mechanism for
the presynaptic targeting of Trio and support a model in which Piccolo and
Bassoon play a role in regulating neurotransmission through interactions with
proteins, including Trio, that modulate the dynamic assembly of F-actin during
cycles of synaptic vesicle exo- and endocytosis.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins
Piccolo and Bassoon
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 11 (2016), 12, Artikel Nr. e0167535
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0167535
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1371/journal.pone.0167535
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000026250
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007619
dcterms.accessRights.openaire
open access