dc.contributor.author
Klassert, Tilman E.
dc.contributor.author
Goyal, Surabhi
dc.contributor.author
Stock, Magdalena
dc.contributor.author
Driesch, Dominik
dc.contributor.author
Hussain, Abid
dc.contributor.author
Berrocal-Almanza, Luis Carlos
dc.contributor.author
Myakala, Rajashekar
dc.contributor.author
Sumanlatha, Gaddam
dc.contributor.author
Valluri, Vijayalakshmi
dc.contributor.author
Ahmed, Niyaz
dc.contributor.author
Schumann, Ralf R.
dc.contributor.author
Flores, Carlos
dc.contributor.author
Slevogt, Hortense
dc.date.accessioned
2018-06-08T10:51:30Z
dc.date.available
2018-05-17T13:49:40.704Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21207
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24503
dc.description.abstract
Tuberculosis (TB) is a multifactorial disease governed by bacterial, host and
environmental factors. On the host side, growing evidence shows the crucial
role that genetic variants play in the susceptibility to Mycobacterium
tuberculosis (Mtb) infection. Such polymorphisms have been described in genes
encoding for different cytokines and pattern recognition receptors (PRR),
including numerous Toll-like receptors (TLRs). In recent years, several
members of the C-type lectin receptors (CTLRs) have been identified as key
PRRs in TB pathogenesis. Nevertheless, studies to date have only addressed
particular genetic polymorphisms in these receptors or their related pathways
in relation with TB. In the present study, we screened the main CTLR gene
clusters as well as CTLR pathway-related genes for genetic variation
associated with pulmonary tuberculosis (PTB). This case-control study
comprised 144 newly diagnosed pulmonary TB patients and 181 healthy controls
recruited at the Bhagwan Mahavir Medical Research Center (BMMRC), Hyderabad,
India. A two-stage study was employed in which an explorative AmpliSeq-based
screening was followed by a validation phase using iPLEX MassARRAY. Our
results revealed one SNP (rs3774275) in MASP1 significantly associated with
PTB in our population (joint analysis p = 0.0028). Furthermore, serum levels
of MASP1 were significantly elevated in TB patients when compared to healthy
controls. Moreover, in the present study we could observe an impact of
increased MASP1 levels on the lectin pathway complement activity in vitro. In
conclusion, our results demonstrate a significant association of MASP1
polymorphism rs3774275 and MASP1 serum levels with the development of
pulmonary TB. The present work contributes to our understanding of host-Mtb
interaction and reinforces the critical significance of mannose-binding lectin
and the lectin-complement pathway in Mtb pathogenesis. Moreover, it proposes a
MASP1 polymorphism as a potential genetic marker for TB resistance.
en
dc.format.extent
11 Seiten
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
C-type lectin receptor
dc.subject
pulmonary tuberculosis
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
AmpliSeq Screening of Genes Encoding the C-Type Lectin Receptors and Their
Signaling Components Reveals a Common Variant in MASP1 Associated with
Pulmonary Tuberculosis in an Indian Population
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Frontiers in Immunology 9 (2018), Art. 242
dcterms.bibliographicCitation.doi
10.3389/fimmu.2018.00242
dcterms.bibliographicCitation.url
http://doi.org/10.3389/fimmu.2018.00242
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000029754
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000009725
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1664-3224