dc.contributor.author
Bormann, N.
dc.contributor.author
Koliszak, A.
dc.contributor.author
Kasper, S.
dc.contributor.author
Schoen, L.
dc.contributor.author
Hilpert, K.
dc.contributor.author
Volkmer, R.
dc.contributor.author
Kikhney, J.
dc.contributor.author
Wildemann, B.
dc.date.accessioned
2018-06-08T10:51:04Z
dc.date.available
2017-05-30T09:43:00.335Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21200
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24496
dc.description.abstract
Infection of bone is a severe complication due to the variety of bacteria
causing it, their resistance against classical antibiotics, the formation of a
biofilm and the difficulty to eradicate it. Antimicrobial peptides (AMPs) are
naturally occurring peptides and promising candidates for treatment of joint
infections. This study aimed to analyze the effect of short artificial
peptides derived from an optimized library regarding (1) antimicrobial effect
on different bacterial species, (2) efficacy on biofilms, and (3) effect on
osteoblast‑like cells. Culturing the AMP-modifications with Escherichia coli,
Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus
(including clinical isolates of MRSA and MSSA) and Staphylococcus epidermidis
identified one candidate that was most effective against all bacteria. This
AMP was also able to reduce biofilm as demonstrated by FISH and
microcalorimetry. Osteoblast viability and differentiation were not negatively
affected by the AMP. A cation concentration comparable to that physiologically
occurring in blood had almost no negative effect on AMP activity and even with
10% serum bacterial growth was inhibited. Bacteria internalized into
osteoblasts were reduced by the AMP. Taken together the results demonstrate a
high antimicrobial activity of the AMP even against bacteria incorporated in a
biofilm or internalized into cells without harming human osteoblasts.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Antimicrobials
dc.subject
Preclinical research
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
A short artificial antimicrobial peptide shows potential to prevent or treat
bone infections
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Scientific Reports. - 7 (2017), Artikel Nr. 1506
dcterms.bibliographicCitation.doi
10.1038/s41598-017-01698-0
dcterms.bibliographicCitation.url
http://www.nature.com/articles/s41598-017-01698-0
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000027087
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000008251
dcterms.accessRights.openaire
open access