dc.contributor.author
Bock,Cristin N.
dc.contributor.author
Babu, Subash
dc.contributor.author
Breloer, Minka
dc.contributor.author
Rajamanickam, Anuradha
dc.contributor.author
Bhootra, Yukthi
dc.contributor.author
Brunn, Marie-Luise
dc.contributor.author
Kühl, Anja A.
dc.contributor.author
Merle, Roswitha
dc.contributor.author
Löhning, Max
dc.contributor.author
Hartmann, Susanne
dc.contributor.author
Rausch, Sebastian
dc.date.accessioned
2018-06-08T10:47:42Z
dc.date.available
2017-07-04T09:32:47.922Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21094
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24391
dc.description.abstract
Infections by the soil-transmitted threadworm Strongyloides stercoralis affect
50-100 million people worldwide, predominantly in tropic and sub-tropic
regions. Here we assessed the T helper cell phenotypes in threadworm-infected
patients and experimental murine infections with focus on CD4+ T cells co-
expressing markers of Th2 and Th1 differentiation. We show that mice infected
with the close relative S. ratti generate strong Th2 responses characterized
by the expansion of CD4+ GATA-3+ cells expressing IL-4/-5/-13 in blood,
spleen, gut-draining lymph nodes, lung and gut tissue. In addition to
conventional Th2 cells, significantly increased frequencies of GATA-3+T-bet+
Th2/1-hybrid cells were detected in all organs and co-expressed Th2- and
Th1-cytokines at intermediate levels. Assessing the phenotype of blood-derived
CD4+ T cells from South Indian patients infected with S. stercoralis and local
uninfected control donors we found that GATA-3 expressing Th2 cells were
significantly increased in the patient cohort, coinciding with elevated
eosinophil and IgE/IgG4 levels. A fraction of IL-4+CD4+ T cells simultaneously
expressed IFN-γ hence displaying a Th2/1 hybrid phenotype. In accordance with
murine Th2/1 cells, human Th2/1 cells expressed intermediate levels of Th2
cytokines. Contrasting their murine counterparts, human Th2/1 hybrids were
marked by high levels of IFN-γ and rather low GATA-3 expression. Assessing the
effector function of murine Th2/1 cells in vitro we found that Th2/1 cells
were qualified for driving the classical activation of macrophages.
Furthermore, Th2/1 cells shared innate, cytokine-driven effector functions
with Th1 cells. Hence, the key findings of our study are that T helper cells
with combined characteristics of Th2 and Th1 cells are integral to immune
responses of helminth-infected mice, but also occur in helminth-infected
humans and we suggest that Th2/1 cells are poised for the instruction of
balanced immune responses during nematode infections.
en
dc.format.extent
15 Seiten
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Th2/1 Hybrid Cells Occurring in Murine and Human Strongyloidiasis Share
Effector Functions of Th1 Cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Front. Cell. Infect. Microbiol. - 7 (2017), Artikel Nr. 261
dc.identifier.sepid
59362
dcterms.bibliographicCitation.doi
10.3389/fcimb.2017.00261
dcterms.bibliographicCitation.url
http://doi.org/10.3389/fcimb.2017.00261
refubium.affiliation
Veterinärmedizin
de
refubium.funding
Inst. Mitgliedschaft bei Frontiers
refubium.funding
Sonstige
refubium.mycore.fudocsId
FUDOCS_document_000000027141
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000008426
dcterms.accessRights.openaire
open access