dc.contributor.author
Skrzypski, Marek
dc.contributor.author
Kolodziejski, Pawel A.
dc.contributor.author
Mergler, Stefan
dc.contributor.author
Khajavi, Noushafarin
dc.contributor.author
Nowak, Krzysztof W.
dc.contributor.author
Strowski, Mathias Z.
dc.contributor.author
Strowski, Mathias Z.
dc.date.accessioned
2018-06-08T10:29:30Z
dc.date.available
2017-01-16T10:41:21.957Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/20509
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-23812
dc.description.abstract
Highly Ca2+ permeable receptor potential channel vanilloid type 6 (TRPV6)
modulates a variety of biological functions including calcium-dependent cell
growth and apoptosis. So far, the role of TRPV6 in controlling growth of
pancreatic neuroendocrine tumour (NET) cells is unknown. In the present study,
we characterize the expression of TRPV6 in pancreatic BON-1 and QGP-1 NET
cells. Furthermore, we evaluate the impact of TRPV6 on intracellular calcium,
the activity of nuclear factor of activated T-cells (NFAT) and proliferation
of BON-1 cells. TRPV6 expression was assessed by real-time PCR and Western
blot. TRPV6 mRNA expression and protein production were down-regulated by
siRNA. Changes in intracellular calcium levels were detected by fluorescence
calcium imaging (fura-2/AM). NFAT activity was studied by NFAT reporter assay;
cell proliferation by bromodeoxyuridine (BrdU), MTT and propidium iodine
staining. TRPV6 mRNA and protein are present in BON-1 and QGP-1 NET-cells.
Down-regulation of TRPV6 attenuates BON-1 cell proliferation. TRPV6 down-
regulation is associated with decreased Ca2+ response pattern and reduced NFAT
activity. In conclusion, TRPV6 is expressed in pancreatic NETs and modulates
cell proliferation via Ca2+-dependent mechanism, which is accompanied by NFAT
activation.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
TRPV6 modulates proliferation of human pancreatic neuroendocrine BON-1 tumour
cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Bioscience Reports. - 36 (2016), 4, Artikel Nr. e00372
dcterms.bibliographicCitation.doi
10.1042/BSR20160106
dcterms.bibliographicCitation.url
http://www.bioscirep.org/content/36/4/e00372
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000026153
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007535
dcterms.accessRights.openaire
open access