dc.contributor.author
Zimmermann, Natalie
dc.contributor.author
Thormann, Verena
dc.contributor.author
Hu, Bo
dc.contributor.author
Köhler, Anne-Britta
dc.contributor.author
Imai-Matsushima, Aki
dc.contributor.author
Locht, Camille
dc.contributor.author
Arnett, Eusondia
dc.contributor.author
Schlesinger, Larry S.
dc.contributor.author
Zoller, Thomas
dc.contributor.author
Schürmann, Mariana
dc.contributor.author
Kaufmann, Stefan H. E.
dc.contributor.author
Wardemann, Hedda
dc.date.accessioned
2018-06-08T10:22:47Z
dc.date.available
2017-01-09T09:59:33.587Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/20335
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-23638
dc.description.abstract
Accumulating evidence from experimental animal models suggests that antibodies
play a protective role against tuberculosis (TB). However, little is known
about the antibodies generated upon Mycobacterium tuberculosis (MTB) exposure
in humans. Here, we performed a molecular and functional characterization of
the human B‐cell response to MTB by generating recombinant monoclonal
antibodies from single isolated B cells of untreated adult patients with acute
pulmonary TB and from MTB‐exposed healthcare workers. The data suggest that
the acute plasmablast response to MTB originates from reactivated memory B
cells and indicates a mucosal origin. Through functional analyses, we
identified MTB inhibitory antibodies against mycobacterial antigens including
virulence factors that play important roles in host cell infection. The
inhibitory activity of anti‐MTB antibodies was directly linked to their
isotype. Monoclonal as well as purified serum IgA antibodies showed MTB
blocking activity independently of Fc alpha receptor expression, whereas IgG
antibodies promoted the host cell infection. Together, the data provide
molecular insights into the human antibody response to MTB and may thereby
facilitate the design of protective vaccination strategies.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Human isotype‐dependent inhibitory antibody responses against Mycobacterium
tuberculosis
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
EMBO Molecular Medicine. - 8 (2016), 11, S. 1325-1339
dcterms.bibliographicCitation.doi
10.15252/emmm.201606330
dcterms.bibliographicCitation.url
http://embomolmed.embopress.org/content/8/11/1325
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000026099
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007487
dcterms.accessRights.openaire
open access