dc.contributor.author
Abdelmegid, Shaimaa
dc.contributor.author
Murugaiyan, Jayaseelan
dc.contributor.author
Abo-Ismail, Mohamed
dc.contributor.author
Caswell, Jeff L.
dc.contributor.author
Kelton, David
dc.contributor.author
Kirby, Gordon M.
dc.date.accessioned
2018-06-08T10:22:38Z
dc.date.available
2018-04-24T13:44:00.574Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/20328
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-23631
dc.description.abstract
Staphylococcus aureus is the most common contagious pathogen associated with
bovine subclinical mastitis. Current diagnosis of S. aureus mastitis is based
on bacteriological culture of milk samples and somatic cell counts, which lack
either sensitivity or specificity. Identification of milk proteins that
contribute to host defense and their variable responses to pathogenic stimuli
would enable the characterization of putative biomarkers of subclinical
mastitis. To accomplish this, milk whey samples from healthy and mastitic
dairy cows were analyzed using a label-free quantitative proteomics approach.
In total, 90 proteins were identified, of which 25 showed significant
differential abundance between healthy and mastitic samples. In silico
functional analyses indicated the involvement of the differentially abundant
proteins in biological mechanisms and signaling pathways related to host
defense including pathogen-recognition, direct antimicrobial function, and the
acute-phase response. This proteomics and bioinformatics analysis not only
facilitates the identification of putative biomarkers of S. aureus subclinical
mastitis but also recapitulates previous findings demonstrating the abundance
of host defense proteins in intramammary infection. All mass spectrometry data
are available via ProteomeXchange with identifier PXD007516.
en
dc.format.extent
14 Seiten
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
milk proteomics
dc.subject
Staphylococcus aureus
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::636 Viehwirtschaft
dc.title
Identification of Host Defense-Related Proteins Using Label-Free Quantitative
Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus
Subclinical Mastitis
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
International Journal of Molecular Sciences 19 (2018), 78
dcterms.bibliographicCitation.doi
10.3390/ijms19010078
dcterms.bibliographicCitation.url
http://doi.org/10.3390/ijms19010078
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Tier- und Umwelthygiene
refubium.mycore.fudocsId
FUDOCS_document_000000029625
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000009667
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1661-6596
dcterms.isPartOf.issn
1422-0067