dc.contributor.author
Hahn, Marc Benjamin
dc.contributor.author
Meyer, Susann
dc.contributor.author
Kunte, Hans-Jörg
dc.contributor.author
Solmun, Tihomir
dc.contributor.author
Sturm, Heinz
dc.date.accessioned
2018-06-08T10:22:20Z
dc.date.available
2018-01-10T13:37:43.648Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/20310
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-23614
dc.description.abstract
The determination of the microscopic dose-damage relationship for DNA in an
aqueous environment is of a fundamental interest for dosimetry and
applications in radiation therapy and protection. We combine geant4 particle-
scattering simulations in water with calculations concerning the movement of
biomolecules to obtain the energy deposit in the biologically relevant
nanoscopic volume. We juxtaposition these results to the experimentally
determined damage to obtain the dose-damage relationship at a molecular level.
This approach is tested for an experimentally challenging system concerning
the direct irradiation of plasmid DNA (pUC19) in water with electrons as
primary particles. Here a microscopic target model for the plasmid DNA based
on the relation of lineal energy and radiation quality is used to calculate
the effective target volume. It was found that on average fewer than two
ionizations within a 7.5-nm radius around the sugar-phosphate backbone are
sufficient to cause a single strand break, with a corresponding median lethal
energy deposit being E1/2=6±4 eV. The presented method is applicable for
ionizing radiation (e.g., γ rays, x rays, and electrons) and a variety of
targets, such as DNA, proteins, or cells.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie
dc.title
Measurements and simulations of microscopic damage to DNA in water by 30 keV
electrons
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Physical Review E. - 95 (2017), 5, Artikel Nr. 052419
dc.title.subtitle
A general approach applicable to other radiation sources and biological
targets
dcterms.bibliographicCitation.doi
10.1103/PhysRevE.95.052419
dcterms.bibliographicCitation.url
http://doi.org/10.1103/PhysRevE.95.052419
refubium.affiliation
Physik
de
refubium.mycore.fudocsId
FUDOCS_document_000000028752
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000009301
dcterms.accessRights.openaire
open access