In this study, we investigated the impact of different land use intensities
(LUI) on the root-associated microbiome of Dactylis glomerata (orchardgrass).
For this purpose, eight sampling sites with different land use intensity
levels but comparable soil properties were selected in the southwest of
Germany. Experimental plots covered land use levels from natural grassland up
to intensively managed meadows. We used 16S rRNA gene based barcoding to
assess the plant-associated community structure in the endosphere, rhizosphere
and bulk soil of D. glomerata. Samples were taken at the reproductive stage of
the plant in early summer. Our data indicated that roots harbor a distinct
bacterial community, which clearly differed from the microbiome of the
rhizosphere and bulk soil. Our results revealed Pseudomonadaceae,
Enterobacteriaceae and Comamonadaceae as the most abundant endophytes
independently of land use intensity. Rhizosphere and bulk soil were dominated
also by Proteobacteria, but the most abundant families differed from those
obtained from root samples. In the soil, the effect of land use intensity was
more pronounced compared to root endophytes leading to a clearly distinct
pattern of bacterial communities under different LUI from rhizosphere and bulk
soil vs. endophytes. Overall, a change of community structure on the
plant–soil interface was observed, as the number of shared OTUs between all
three compartments investigated increased with decreasing land use intensity.
Thus, our findings suggest a stronger interaction of the plant with its
surrounding soil under low land use intensity. Furthermore, the amount and
quality of available nitrogen was identified as a major driver for shifts in
the microbiome structure in all compartments.
land use change
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie
The Influence of Land Use Intensity on the Plant-Associated Microbiome of
Dactylis glomerata L.
Front. Plant Sci. - 8 (2017), Artikel Nr. 930
Biologie, Chemie, Pharmazie