dc.contributor.author
Linke, Martin
dc.contributor.author
Yang, Yang
dc.contributor.author
Zienicke, Benjamin
dc.contributor.author
Hammam, Mostafa A. S.
dc.contributor.author
Haimberger, Theodore von
dc.contributor.author
Zacarias, Angelica
dc.contributor.author
Inomata, Katsuhiko
dc.contributor.author
Lamparter, Tilman
dc.contributor.author
Heyne, Karsten
dc.date.accessioned
2018-06-08T07:17:08Z
dc.date.available
2014-07-08T06:44:16.566Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/17579
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-21463
dc.description.abstract
Photoisomerization of biliverdin (BV) chromophore triggers the photoresponse
in native Agp1 bacteriophytochrome. We discuss heterogeneity in phytochrome Pr
form to account for the shape of the absorption profile. We investigated
different regions of the absorption profile by angle balanced polarization
resolved femtosecond VIS pump–IR probe spectroscopy. We studied the Pr form of
Agp1 with its natural chromophore and with a sterically locked 18Et-BV (locked
Agp1). We followed the dynamics and orientations of the carbonyl stretching
vibrations of ring D and ring A in their ground and electronically excited
states. Photoisomerization of ring D is reflected by strong signals of the
ring D carbonyl vibration. In contrast, orientational data on ring A show no
rotation of ring A upon photoexcitation. Orientational data allow excluding a
ZZZasa geometry and corroborates a nontwisted ZZZssa geometry of the
chromophore. We found no proof for heterogeneity but identified a new, to our
knowledge, electronic transition in the absorption profile at 644 nm (S0→S2).
Excitation of the S0→S2 transition will introduce a more complex photodynamics
compared with S0→S1 transition. Our approach provides fundamental information
on disentanglement of absorption profiles, identification of chromophore
structures, and determination of molecular groups involved in the
photoisomerization process of photoreceptors.
de
dc.rights.uri
http://creativecommons.org/licenses/by-nc/2.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik
dc.title
Electronic transitions and heterogeneity of the bacteriophytochrome Pr
absorption band
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Biophysical Journal. - 105 (2013), 8, S. 1756–1766
dc.identifier.sepid
33183
dc.title.subtitle
An angle balanced polarization resolved femtosecond VIS pump–IR probe study
dcterms.bibliographicCitation.doi
10.1016/j.bpj.2013.08.041
dcterms.bibliographicCitation.url
http://www.cell.com/biophysj/abstract/S0006-3495%2813%2900988-0
refubium.affiliation
Physik
de
refubium.mycore.fudocsId
FUDOCS_document_000000020563
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000003683
dcterms.accessRights.openaire
open access