dc.contributor.author
Schütze, Tatjana
dc.contributor.author
Wilhelm, Barbara
dc.contributor.author
Greiner, Nicole
dc.contributor.author
Braun, Hannsjörg
dc.contributor.author
Peter, Franziska
dc.contributor.author
Mörl, Mario
dc.contributor.author
Erdmann, Volker A.
dc.contributor.author
Lehrach, Hans
dc.contributor.author
Konthur, Zoltán
dc.contributor.author
Menger, Marcus
dc.contributor.author
Arndt, Peter F.
dc.contributor.author
Glökler, Jörn
dc.date.accessioned
2018-06-08T07:17:01Z
dc.date.available
2015-11-23T11:02:06.882Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/17575
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-21459
dc.description.abstract
Background SELEX is an iterative process in which highly diverse synthetic
nucleic acid libraries are selected over many rounds to finally identify
aptamers with desired properties. However, little is understood as how binders
are enriched during the selection course. Next-generation sequencing offers
the opportunity to open the black box and observe a large part of the
population dynamics during the selection process. Methodology We have
performed a semi-automated SELEX procedure on the model target streptavidin
starting with a synthetic DNA oligonucleotide library and compared results
obtained by the conventional analysis via cloning and Sanger sequencing with
next-generation sequencing. In order to follow the population dynamics during
the selection, pools from all selection rounds were barcoded and sequenced in
parallel. Conclusions High affinity aptamers can be readily identified simply
by copy number enrichment in the first selection rounds. Based on our results,
we suggest a new selection scheme that avoids a high number of iterative
selection rounds while reducing time, PCR bias, and artifacts.
en
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/de/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie
dc.title
Probing the SELEX Process with Next-Generation Sequencing
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 6 (2011), 12, Artikel Nr. e29604
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0029604
dcterms.bibliographicCitation.url
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0029604
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000023508
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005696
dcterms.accessRights.openaire
open access