dc.contributor.author
Ashktorab, Hassan
dc.contributor.author
Hermann, Pia
dc.contributor.author
Nouraie, Mehdi
dc.contributor.author
Shokrani, Babak
dc.contributor.author
Lee, Edward
dc.contributor.author
Haidary, Tahmineh
dc.contributor.author
Brim, Hassan
dc.contributor.author
Stein, Ulrike
dc.date.accessioned
2018-06-08T04:20:34Z
dc.date.available
2016-09-20T09:31:59.651Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/17089
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-21269
dc.description.abstract
Background Colorectal cancer is a preventable disease if caught at early
stages. This disease is highly aggressive and has a higher incidence in
African Americans. Several biomarkers and mutations of aggressive tumor
behavior have been defined such as metastasis-associated in colon cancer 1
(MACC1) that was associated with metastasis in colorectal cancer patients.
Here, we aim to assess colon tissue MACC1 protein and circulating MACC1
transcripts in colon preneoplastic and neoplastic African American patients.
Methods Patients’ tissue samples (n = 143) have been arranged on three tissue
microarrays for normal (n = 26), adenoma (n = 68) and cancer (n = 49) samples.
Immunohistochemistry was used to detect MACC1 expression. Blood samples (n =
93) from normal (n = 45), hyperplastic (n = 15) and tubular adenoma (n = 33)
patients were used to assess MACC1 transcripts using qRT-PCR. Distribution of
continuous variables was tested between different diagnoses with
Kruskal–Wallis test. Categorical variables were tested by Chi square test. We
assessed the prognostic ability of IHC staining by calculating area under
receiver operating characteristics curve (ROC) for adenoma and cancer
separately. Differences between groups in terms of MACC1 transcript levels in
plasma were calculated by using non-parametric (exact) Wilcoxon-Mann–Whitney
tests. We performed all calculations with SPSS, version 21. Results In patient
tissues, there was a statistically significant difference in MACC1 expression
in normal vs. adenoma samples (p = 0.004) and normal vs. cancer samples (p <
0.001). There was however no major difference in MACC1 expression between
adenoma vs. cancer cases or tubular adenomas vs tubulovillous adenomas. The
area under the curve for both normal vs. adenoma and normal vs. cancer cases
were 70 and 67 %, respectively. MACC1 expression was not correlated to age,
gender or anatomical sample location. In patient plasma, MACC1 transcripts in
adenoma patients were significantly higher than in plasma from normal patients
(p = 0.014). However, the difference between normal and hyperplastic plasma
MACC1 transcripts was not statistically significant. Conclusion Metastasis-
associated in colon cancer 1 is expressed at early stages of colorectal
oncogenesis within the affected colonic tissue in this patient cohort. The
plasma transcripts can be used to stratify African American patients at risk
for potential malignant colonic lesions.
de
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Colorectal cancer
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Increased MACC1 levels in tissues and blood identify colon adenoma patients at
high risk
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Journal of Translational Medicine. - 14 (2016), Artikel Nr. 215
dcterms.bibliographicCitation.doi
10.1186/s12967-016-0971-0
dcterms.bibliographicCitation.url
http://translational-medicine.biomedcentral.com/articles/10.1186/s12967-016-0971-0
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000025400
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006988
dcterms.accessRights.openaire
open access