dc.contributor.author
Witzel, Christian
dc.contributor.author
Reutter, Werner
dc.contributor.author
Stark, G. Björn
dc.contributor.author
Koulaxouzidis, Georgios
dc.date.accessioned
2018-06-08T04:16:49Z
dc.date.available
2015-07-28T10:10:15.345Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16964
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-21145
dc.description.abstract
Increasing evidence indicates that sialic acid plays an important role during
nerve regeneration. Sialic acids can be modified in vitro as well as in vivo
using metabolic oligosaccharide engineering of the N-acyl side chain.
N-Propionylmannosamine (ManNProp) increases neurite outgrowth and accelerates
the reestablishment of functional synapses in vitro. We investigated the
influence of systemic ManNProp application using a specific in vivo mouse
model. Using mice expressing axonal fluorescent proteins, we quantified the
extension of regenerating axons, the number of regenerating axons, the number
of arborising axons and the number of branches per axon 5 days after injury.
Sciatic nerves from non-expressing mice were grafted into those expressing
yellow fluorescent protein. We began a twice-daily intraperitoneal application
of either peracetylated ManNProp (200 mg/kg) or saline solution 5 days before
injury, and continued it until nerve harvest (5 days after transection).
ManNProp significantly increased the mean distance of axonal regeneration
(2.49 mm vs. 1.53 mm; P < 0.005) and the number of arborizing axons (21% vs.
16%; P = 0.008) 5 days after sciatic nerve grafting. ManNProp did not affect
the number of regenerating axons or the number of branches per arborizing
axon. The biochemical glycoengineering of the N-acyl side chain of sialic acid
might be a promising approach for improving peripheral nerve regeneration.
de
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subject
N-propionylmannosamine
dc.subject
glycoengineering
dc.subject
peripheral nerve
dc.subject
Thy1-YFP mouse
dc.subject
nerve regeneration
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
N-Propionylmannosamine stimulates axonal elongation in a murine model of
sciatic nerve injury
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Neural Regeneration Research. - 10 (2015), 6, S. 976-981
dcterms.bibliographicCitation.doi
10.4103/1673-5374.150744
dcterms.bibliographicCitation.url
http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=6;spage=976;epage=981;aulast=Witzel
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000022891
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005253
dcterms.accessRights.openaire
open access