dc.contributor.author
Mayer-Scholl, Anne
dc.contributor.author
Murugaiyan, Jayaseelan
dc.contributor.author
Neumann, Jennifer
dc.contributor.author
Bahn, Peter
dc.contributor.author
Reckinger, Sabine
dc.contributor.author
Nöckler, Karsten
dc.date.accessioned
2018-06-08T04:16:12Z
dc.date.available
2016-04-21T08:28:05.591Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16940
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-21121
dc.description.abstract
Human trichinellosis occurs through consumption of raw or inadequately
processed meat or meat products containing larvae of the parasitic nematodes
of the genus Trichinella. Currently, nine species and three genotypes are
recognized, of which T. spiralis, T. britovi and T. pseudospiralis have the
highest public health relevance. To date, the differentiation of the larvae to
the species and genotype level is based primarily on molecular methods, which
can be relatively time consuming and labor intensive. Due to its rapidness and
ease of use a matrix assisted laser desorption / ionization time of flight
mass spectrometry (MALDI-TOF MS) reference spectra database using Trichinella
strains of all known species and genotypes was created. A
formicacid/acetonitrile protein extraction was carried out after pooling 10
larvae of each Trichinella species and genotype. Each sample was spotted 9
times using α-cyano 4-hydoxy cinnamic acid matrix and a MicroFlex LT mass
spectrometer was used to acquire 3 spectra (m/z 2000 to 20000 Da) from each
spot resulting in 27 spectra/species or genotype. Following the spectra
quality assessment, Biotyper software was used to create a main spectra
library (MSP) representing nine species and three genotypes of Trichinella.
The evaluation of the spectra generated by MALDI-TOF MS revealed a
classification which was comparable to the results obtained by molecular
methods. Also, each Trichinella species utilized in this study was distinct
and distinguishable with a high confidence level. Further, different
conservation methods such as freezing and conservation in alcohol and the host
species origin of the isolated larvae did not have a significant influence on
the generated spectra. Therefore, the described MALDI-TOF MS can successfully
be implemented for both genus and species level identification and represents
a major step forward in the use of this technique in foodborne parasitology.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie
dc.title
Rapid Identification of the Foodborne Pathogen Trichinella spp. by Matrix-
Assisted Laser Desorption/Ionization Mass Spectrometry
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 11 (2016), 3, Artikel Nr. e0152062
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0152062
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1371/journal.pone.0152062
refubium.affiliation
Veterinärmedizin
de
refubium.mycore.fudocsId
FUDOCS_document_000000024408
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006317
dcterms.accessRights.openaire
open access