dc.contributor.author
Baleela, Rania
dc.contributor.author
Llewellyn, Martin S.
dc.contributor.author
Fitzpatrick, Sinead
dc.contributor.author
Kuhls, Katrin
dc.contributor.author
Schönian, Gabriele
dc.contributor.author
Miles, Michael A.
dc.contributor.author
Mauricio, Isabel L.
dc.date.accessioned
2018-06-08T04:04:24Z
dc.date.available
2015-01-12T10:18:22.957Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16523
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20704
dc.description.abstract
Background Visceral leishmaniasis (VL), caused by the members of the
Leishmania donovani complex, has been responsible for devastating VL epidemics
in the Sudan. Multilocus microsatellite and sequence typing studies can
provide valuable insights into the molecular epidemiology of leishmaniasis,
when applied at local scales. Here we present population genetic data for a
large panel of strains and clones collected in endemic Sudan between 1993 and
2001. Methods Genetic diversity was evaluated at fourteen microsatellite
markers and eleven nuclear sequence loci across 124 strains and clones.
Results Microsatellite data defined six genetic subpopulations with which the
nuclear sequence data were broadly congruent. Pairwise estimates of FST
(microsatellite) and KST (sequence) indicated small but significant shifts
among the allelic repertoires of circulating strains year on year.
Furthermore, we noted the co-occurrence of human and canine L. donovani
strains in three of the six clusters defined. Finally, we identified
widespread deficit in heterozygosity in all four years tested but strong
deviation from inter-locus linkage equilibrium in two years. Conclusions
Significant genetic diversity is present among L. donovani in Sudan, and minor
population structuring between years is characteristic of entrenched, endemic
disease transmission. Seasonality in vector abundance and transmission may, to
an extent, explain the shallow temporal clines in allelic frequency that we
observed. Genetically similar canine and human strains highlight the role of
dogs as important local reservoirs of visceral leishmaniasis.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Leishmania donovani populations in Eastern Sudan
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Parasites & Vectors. 7 (2014), 1, Artikel Nr. 496
dc.title.subtitle
temporal structuring and a link between human and canine transmission
dcterms.bibliographicCitation.doi
10.1186/s13071-014-0496-4
dcterms.bibliographicCitation.url
http://www.parasitesandvectors.com/content/7/1/496
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000021562
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004349
dcterms.accessRights.openaire
open access