dc.contributor.author
Goodwin, Thomas J.
dc.contributor.author
McCarthy, Maureen
dc.contributor.author
Osterrieder, Nikolaus
dc.contributor.author
Cohrs, Randall J.
dc.contributor.author
Kaufer, Benedikt B.
dc.date.accessioned
2018-06-08T04:01:33Z
dc.date.available
2014-01-24T22:15:00.921Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16436
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20617
dc.description.abstract
Varicella-zoster virus (VZV) is a neurotropic human alphaherpesvirus that
causes varicella upon primary infection, establishes latency in multiple
ganglionic neurons, and can reactivate to cause zoster. Live attenuated VZV
vaccines are available; however, they can also establish latent infections and
reactivate. Studies of VZV latency have been limited to the analyses of human
ganglia removed at autopsy, as the virus is strictly a human pathogen.
Recently, terminally differentiated human neurons have received much attention
as a means to study the interaction between VZV and human neurons; however,
the short life-span of these cells in culture has limited their application.
Herein, we describe the construction of a model of normal human neural
progenitor cells (NHNP) in tissue-like assemblies (TLAs), which can be
successfully maintained for at least 180 days in three-dimensional (3D)
culture, and exhibit an expression profile similar to that of human trigeminal
ganglia. Infection of NHNP TLAs with cell-free VZV resulted in a persistent
infection that was maintained for three months, during which the virus genome
remained stable. Immediate-early, early and late VZV genes were transcribed,
and low-levels of infectious VZV were recurrently detected in the culture
supernatant. Our data suggest that NHNP TLAs are an effective system to
investigate long-term interactions of VZV with complex assemblies of human
neuronal cells.
de
dc.rights.uri
https://creativecommons.org/publicdomain/zero/1.0/deed.de
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
Three-dimensional normal human neural progenitor tissue-like assemblies: A model of persistent Varicella-Zoster virus infection
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Plos Pathogens August 2013 | Volume 9 | Issue 8 | e100351
dc.contributor.contact
b.kaufer@fu-berlin.de
dcterms.bibliographicCitation.doi
10.1371/journal.ppat.1003512
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1371/journal.ppat.1003512
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Virologie
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refubium.funding
Deutsche Forschungsgemeinschaft (DFG)
refubium.mycore.fudocsId
FUDOCS_document_000000018673
refubium.note.author
Gefördert durch die DFG und den Open-Access-Publikationsfonds der Freien
Universität Berlin
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000002744
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1553-7374
dcterms.isPartOf.issn
1553-7366