dc.contributor.author
Abdel-latief, Mohatmed
dc.date.accessioned
2018-06-08T04:01:17Z
dc.date.available
2015-11-26T11:20:12.128Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16420
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20601
dc.description.abstract
Chemoperception in invertebrates is mediated by a family of G-protein-coupled
receptors (GPCR). To date nothing is known about the molecular mechanisms of
chemoperception in coleopteran species. Recently the genome of Tribolium
castaneum was sequenced for use as a model species for the Coleoptera. Using
blast searches analyses of the T. castaneum genome with previously predicted
amino acid sequences of insect chemoreceptor genes, a putative chemoreceptor
family consisting of 62 gustatory receptors (Grs) and 26 olfactory receptors
(Ors) was identified. The receptors have seven transmembrane domains (7TMs)
and all belong to the GPCR receptor family. The expression of the T. castaneum
chemoreceptor genes was investigated using quantification real- time RT-PCR
and in situ whole mount RT-PCR analysis in the antennae, mouth parts, and
prolegs of the adults and larvae. All of the predicted TcasGrs were expressed
in the labium, maxillae, and prolegs of the adults but TcasGr13, 19, 28, 47,
62, 98, and 61 were not expressed in the prolegs. The TcasOrs were localized
only in the antennae and not in any of the beetles gustatory organs with one
exception; the TcasOr16 (like DmelOr83b), which was localized in the antennae,
labium, and prolegs of the beetles. A group of six TcasGrs that presents a
lineage with the sugar receptors subfamily in Drosophila melanogaster were
localized in the lacinia of the Tribolium larvae. TcasGr1, 3, and 39,
presented an ortholog to CO2 receptors in D. melanogaster and Anopheles
gambiae was recorded. Low expression of almost all of the predicted
chemoreceptor genes was observed in the head tissues that contain the brains
and suboesophageal ganglion (SOG). These findings demonstrate the
identification of a chemoreceptor family in Tribolium, which is evolutionarily
related to other insect species.
de
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/de/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)
dc.title
A Family of Chemoreceptors in Tribolium castaneum (Tenebrionidae: Coleoptera)
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 2 (2007), 12, Artikel Nr. e1319
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0001319
dcterms.bibliographicCitation.url
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0001319
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000023538
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005725
dcterms.accessRights.openaire
open access