dc.contributor.author
Siebert, Matthias
dc.contributor.author
Böhme, Mathias A.
dc.contributor.author
Driller, Jan H.
dc.contributor.author
Babikir, Husam
dc.contributor.author
Mampell, Malou M.
dc.contributor.author
Rey, Ulises
dc.contributor.author
Ramesh, Niraja
dc.contributor.author
Matkovic, Tanja
dc.contributor.author
Holton, Nicole
dc.contributor.author
Reddy-Alla, Suneel
dc.contributor.author
Göttfert, Fabian
dc.contributor.author
Kamin, Dirk
dc.contributor.author
Quentin, Christine
dc.contributor.author
Klinedinst, Susan
dc.contributor.author
Andlauer, Till F. M.
dc.contributor.author
Hell, Stefan W.
dc.contributor.author
Collins, Catherine A.
dc.contributor.author
Wahl, Markus C.
dc.contributor.author
Loll, Bernhard
dc.contributor.author
Sigrist, Stephan J.
dc.date.accessioned
2018-06-08T04:01:11Z
dc.date.available
2015-09-07T08:31:29.116Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16412
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20593
dc.description.abstract
Synaptic vesicles (SVs) fuse at active zones (AZs) covered by a protein
scaffold, at Drosophila synapses comprised of ELKS family member Bruchpilot
(BRP) and RIM-binding protein (RBP). We here demonstrate axonal co-transport
of BRP and RBP using intravital live imaging, with both proteins co-
accumulating in axonal aggregates of several transport mutants. RBP, via its
C-terminal Src-homology 3 (SH3) domains, binds Aplip1/JIP1, a transport
adaptor involved in kinesin-dependent SV transport. We show in atomic detail
that RBP C-terminal SH3 domains bind a proline-rich (PxxP) motif of
Aplip1/JIP1 with submicromolar affinity. Pointmutating this PxxP motif
provoked formation of ectopic AZ-like structures at axonal membranes. Direct
interactions between AZ proteins and transport adaptors seem to provide
complex avidity and shield synaptic interaction surfaces of pre-assembled
scaffold protein transport complexes, thus, favouring physiological synaptic
AZ assembly over premature assembly at axonal membranes. - See more at:
http://elifesciences.org/content/4/e06935#sthash.oVGZ8cdi.dpuf
de
dc.rights.uri
http://www.fu-berlin.de/sites/refubium/rechtliches/Nutzungsbedingungen
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
A high affinity RIM-binding protein/Aplip1 interaction prevents the formation
of ectopic axonal active zones
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
eLife. - 4 (2015), Artikel Nr. e06935
dcterms.bibliographicCitation.doi
10.7554/eLife.06935
dcterms.bibliographicCitation.url
http://dx.doi.org/10.7554/eLife.06935
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000023063
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005371
dcterms.accessRights.openaire
open access