dc.contributor.author
Lissek, Thomas
dc.contributor.author
Obenhaus, Horst A.
dc.contributor.author
Ditzel, Desiree A. W.
dc.contributor.author
Nagai, Takeharu
dc.contributor.author
Miyawaki, Atsushi
dc.contributor.author
Sprengel, Rolf
dc.contributor.author
Hasan, Mazahir T.
dc.date.accessioned
2018-06-08T03:58:31Z
dc.date.available
2016-05-09T09:31:22.138Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16325
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20508
dc.description.abstract
General anesthetics are commonly used in animal models to study how sensory
signals are represented in the brain. Here, we used two-photon (2P) calcium
activity imaging with cellular resolution to investigate how neuronal activity
in layer 2/3 of the mouse barrel cortex is modified under the influence of
different concentrations of chemically distinct general anesthetics. Our
results show that a high isoflurane dose induces synchrony in local neuronal
networks and these cortical activity patterns closely resemble those observed
in EEG recordings under deep anesthesia. Moreover, ketamine and urethane also
induced similar activity patterns. While investigating the effects of deep
isoflurane anesthesia on whisker and auditory evoked responses in the barrel
cortex, we found that dedicated spatial regions for sensory signal processing
become disrupted. We propose that our isoflurane-2P imaging paradigm can serve
as an attractive model system to dissect cellular and molecular mechanisms
that induce the anesthetic state, and it might also provide important insight
into sleep-like brain states and consciousness.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
cerebral cortex
dc.subject
two-photon imaging
dc.subject
genetically encoded calcium indicators
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
General Anesthetic Conditions Induce Network Synchrony and Disrupt Sensory
Processing in the Cortex
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Front. Cell. Neurosci. - 10 (2016), Artikel Nr. 64
dcterms.bibliographicCitation.doi
10.3389/fncel.2016.00064
dcterms.bibliographicCitation.url
http://dx.doi.org/10.3389/fncel.2016.00064
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000024482
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006371
dcterms.accessRights.openaire
open access