dc.contributor.author
Grabowski, Katja
dc.contributor.author
Riemenschneider, Mona
dc.contributor.author
Schulte, Leonard
dc.contributor.author
Witten, Anika
dc.contributor.author
Schulz, Angela
dc.contributor.author
Stoll, Monika
dc.contributor.author
Kreutz, Reinhold
dc.date.accessioned
2018-06-08T03:55:06Z
dc.date.available
2015-03-13T11:27:59.598Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16203
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20387
dc.description.abstract
Reactivation of fetal gene expression patterns has been implicated in common
cardiac diseases in adult life including left ventricular (LV) hypertrophy
(LVH) in arterial hypertension. Thus, increased wall stress and neurohumoral
activation are discussed to induce the return to expression of fetal genes
after birth in LVH. We therefore aimed to identify novel potential candidates
for LVH by analyzing fetal-adult cardiac gene expression in a genetic rat
model of hypertension, i.e. the stroke-prone spontaneously hypertensive rat
(SHRSP). To this end we performed genome-wide transcriptome analysis in SHRSP
to identify differences in expression patterns between day 20 of fetal
development (E20) and adult animals in week 14 in comparison to a normotensive
rat strain with contrasting low LV mass, i.e. Fischer (F344). 15232 probes
were detected as expressed in LV tissue obtained from rats at E20 and week 14
(p < 0.05) and subsequently screened for differential expression. We
identified 24 genes with SHRSP specific up-regulation and 21 genes with down-
regulation as compared to F344. Further bioinformatic analysis presented
Efcab6 as a new candidate for LVH that showed only in the hypertensive SHRSP
rat differential expression during development (logFC = 2.41, p < 0.001) and
was significantly higher expressed in adult SHRSP rats compared with adult
F344 (+ 76%) and adult normotensive Wistar-Kyoto rats (+ 82%). Thus, it
represents an interesting new target for further functional analyses and the
elucidation of mechanisms leading to LVH. Here we report a new approach to
identify candidate genes for cardiac hypertrophy by combining the analysis of
gene expression differences between strains with a contrasting cardiac
phenotype with a comparison of fetal-adult cardiac expression patterns.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik
dc.title
Fetal-Adult Cardiac Transcriptome Analysis in Rats with Contrasting Left
Ventricular Mass Reveals New Candidates for Cardiac Hypertrophy
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 10 (2015), 2, Artikel Nr. e0116807
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0116807
dcterms.bibliographicCitation.url
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116807
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000022044
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004671
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1932-6203