dc.contributor.author
Richter, C.
dc.contributor.author
Schneider, C.
dc.contributor.author
Quick, M. T.
dc.contributor.author
Volz, Pierre
dc.contributor.author
Mahrwald, R.
dc.contributor.author
Hughes, J.
dc.contributor.author
Dick, B.
dc.contributor.author
Alexiev, Ulrike
dc.contributor.author
Ernsting, N. P.
dc.date.accessioned
2018-06-08T03:54:49Z
dc.date.available
2016-03-14T12:16:23.319Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16196
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20380
dc.description.abstract
Although seminaphtorhodafluor (SNARF) dyes are already widely used to measure
pH in cells and at biofilms, their synthesis has low yield and results in an
unspecific position of a carboxy-group. The separation of 5′- and 6′-carboxy-
SNARF reveals a pKa difference of 0.15, calling into question pH measurements
with the (commercially available) mixture. Here we replace the bulky external
dicarboxyphenyl ring with a propionate group and evaluate the spectral
properties of the new derivative. Proceeding to the ethyl-iodoacetamide,
covalent linkage to cysteine protein sites is achieved efficiently as shown
with a cyanobacterial phytochrome, extending the scarce application of SNARF
in bio-labelling in the current literature. Application in fluorescence
lifetime imaging is demonstrated both with the lifetime-based and ratiometric-
yield method.
en
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie
dc.title
Dual-fluorescence pH probe for bio-labelling
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Phys. Chem. Chem. Phys. - 17 (2015), 45, S. 30590-30597
dc.identifier.sepid
49530
dcterms.bibliographicCitation.doi
10.1039/c5cp05454k
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1039/C5CP05454K
refubium.affiliation
Physik
de
refubium.affiliation.other
Institut für Experimentalphysik
refubium.mycore.fudocsId
FUDOCS_document_000000024148
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006100
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1463-9076