dc.contributor.author
Hauser, Elisabeth
dc.contributor.author
Mellmann, Alexander
dc.contributor.author
Semmler, Torsten
dc.contributor.author
Stoeber, Helen
dc.contributor.author
Wieler, Lothar Heinz
dc.contributor.author
Karch, Helge
dc.contributor.author
Kuebler, Nikole
dc.contributor.author
Fruth, Angelika
dc.contributor.author
Harmsen, Dag
dc.contributor.author
Weniger, Thomas
dc.contributor.author
Tietze, Erhard
dc.contributor.author
Schmidt, Herbert
dc.date.accessioned
2018-06-08T03:53:52Z
dc.date.available
2014-03-04T09:55:08.323Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16169
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20353
dc.description.abstract
Seventy-five food-associated Shiga toxin-producing Escherichia coli (STEC)
strains were analyzed by molecular and phylogenetic methods to describe their
pathogenic potential. The presence of the locus of proteolysis activity (LPA),
the chromosomal pathogenicity island (PAI) PAI ICL3, and the autotransporter-
encoding gene sabA was examined by PCR. Furthermore, the occupation of the
chromosomal integration sites of the locus of enterocyte effacement (LEE),
selC, pheU, and pheV, as well as the Stx phage integration sites yehV, yecE,
wrbA, z2577, and ssrA, was analyzed. Moreover, the antibiotic resistance
phenotypes of all STEC strains were determined. Multilocus sequence typing
(MLST) was performed, and sequence types (STs) and sequence type complexes
(STCs) were compared with those of 42 hemolytic-uremic syndrome
(HUS)-associated enterohemorrhagic E. coli (HUSEC) strains. Besides 59 STs and
4 STCs, three larger clusters were defined in this strain collection. Clusters
A and C consist mostly of highly pathogenic eae-positive HUSEC strains and
some related food-borne STEC strains. A member of a new O26 HUS-associated
clone and the 2011 outbreak strain E. coli O104:H4 were found in cluster A.
Cluster B comprises only eae-negative food-borne STEC strains as well as
mainly eae-negative HUSEC strains. Although food-borne strains of cluster B
were not clearly associated with disease, serotypes of important pathogens,
such as O91:H21 and O113:H21, were in this cluster and closely related to the
food-borne strains. Clonal analysis demonstrated eight closely related genetic
groups of food-borne STEC and HUSEC strains that shared the same ST and were
similar in their virulence gene composition. These groups should be considered
with respect to their potential for human infection.
en
dc.rights.uri
http://journals.asm.org/site/misc/ASM_Author_Statement.xhtml
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
Phylogenetic and Molecular Analysis of Food-Borne Shiga Toxin-Producing
Escherichia coli
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Applied and Environmental Microbiology. - 79 (2013), 8, S.2731-2740
dc.identifier.sepid
32213
dcterms.bibliographicCitation.doi
10.1128/AEM.03552-12
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1128/AEM.03552-12
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Mikrobiologie und Tierseuchen
refubium.mycore.fudocsId
FUDOCS_document_000000019662
refubium.note.author
Verlags-PDF darf archiviert werden, siehe:
http://www.sherpa.ac.uk/romeo/search.php
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000003080
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
0099-2240