dc.contributor.author
Mulsow, Katharina
dc.contributor.author
Eidenschink, Juliane
dc.contributor.author
Melzig, Matthias F.
dc.date.accessioned
2018-06-08T03:52:52Z
dc.date.available
2015-06-25T08:16:12.015Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/16124
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20308
dc.description.abstract
Due to increasing health consciousness, a lot of food supplements are sold and
used. Dietary supplements of Glycine max (L.) MERR. are used as an alternative
treatment for menopausal complaints such as hot flashes. Thereby, the
effective soy compounds are the isoflavones daidzin, genistin, and glycitin.
However, only the total soy extract content of the nutritional supplements is
indicated. The aim of this study is to introduce a fast, efficient, and
economic Fourier transformation infrared (FT-IR) spectroscopy method to
quantify the active ingredients in the complex matrix of soy-based
supplements. Five different nutritional supplements of Glycine max (L.) MERR.
were purchased from a German pharmacy and were extracted with 80% aqueous
methanol. A high-performance liquid chromatography (HPLC) method was used for
the separation. The samples were concentrated and measured with infrared
spectroscopy. An FT-IR method was established to quantify the active
ingredients in the complex matrix of soy-based nutritional supplements. The
partial least-squares algorithm was used to develop the method, which enabled
the estimation of the content of particular isoflavones (daidzin R² = 0.86,
glycitin R² = 0.94, genistin R² = 0.96) and the quantification of the total
isoflavone content (R² = 0.92) despite peak overlap in the infrared (IR)
spectra. The method for the quantification of the isoflavonol glycosides is
precise with the standard error of prediction being 13.54%.
de
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Glycine max (L.) MERR.
dc.subject
Fourier Transformation Infrared
dc.subject
Quantification
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik
dc.title
FT-IR Method for the Quantification of Isoflavonol Glycosides in Nutritional
Supplements of Soy (Glycine max (L.) MERR.)
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Scientia Pharmaceutica. - 83 (2015), 2, S. 377-386
dcterms.bibliographicCitation.doi
10.3797/scipharm.1410-02
dcterms.bibliographicCitation.url
http://dx.doi.org/10.3797/scipharm.1410-02
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000022695
refubium.note.author
Der Artikel wurde in einer Open-Access-Artikel publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005097
dcterms.accessRights.openaire
open access