dc.contributor.author
Gonzalez-Tortuero, Enrique
dc.contributor.author
Rusek, Jakub
dc.contributor.author
Maayan, Inbar
dc.contributor.author
Petrusek, Adam
dc.contributor.author
Pialek, Lubomir
dc.contributor.author
Laurent, Stefan
dc.contributor.author
Wolinska, Justyna
dc.date.accessioned
2018-06-08T03:47:19Z
dc.date.available
2016-07-12T11:43:06.823Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15928
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20115
dc.description.abstract
Background Microsporidia are spore-forming obligate intracellular parasites
that include both emerging pathogens and economically important disease
agents. However, little is known about the genetic diversity of microsporidia.
Here, we investigated patterns of geographic population structure,
intraspecific genetic variation, and recombination in two microsporidian taxa
that commonly infect cladocerans of the Daphnia longispina complex in central
Europe. Taken together, this information helps elucidate the reproductive mode
and life-cycles of these parasite species. Methods Microsporidia-infected
Daphnia were sampled from seven drinking water reservoirs in the Czech
Republic. Two microsporidia species (Berwaldia schaefernai and microsporidium
lineage MIC1) were sequenced at the internal transcribed spacer (ITS) region,
using the 454 pyrosequencing platform. Geographical structure analyses were
performed applying Fisher’s exact tests, analyses of molecular variance, and
permutational MANOVA. To evaluate the genetic diversity of the ITS region, the
number of polymorphic sites and Tajima’s and Watterson’s estimators of theta
were calculated. Tajima’s D was also used to determine if the ITS in these
taxa evolved neutrally. Finally, neighbour similarity score and pairwise
homology index tests were performed to detect recombination events. Results
While there was little variation among Berwaldia parasite strains infecting
different host populations, the among-population genetic variation of MIC1 was
significant. Likewise, ITS genetic diversity was lower in Berwaldia than in
MIC1. Recombination signals were detected only in Berwaldia. Conclusion
Genetic tests showed that parasite populations could have expanded recently
after a bottleneck or that the ITS could be under negative selection in both
microsporidia species. Recombination analyses might indicate cryptic sex in
Berwaldia and pure asexuality in MIC1. The differences observed between the
two microsporidian species present an exciting opportunity to study the
genetic basis of microsporidia-Daphnia coevolution in natural populations, and
to better understand reproduction in these parasites.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Genetic diversity
dc.subject
Internal transcribed spacer
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie
dc.title
Genetic diversity of two Daphnia-infecting microsporidian parasites, based on
sequence variation in the internal transcribed spacer region
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Parasites & Vectors. - 9 (2016), Artikel Nr. 293
dcterms.bibliographicCitation.doi
10.1186/s13071-016-1584-4
dcterms.bibliographicCitation.url
http://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1584-4
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000024988
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006762
dcterms.accessRights.openaire
open access