dc.contributor.author
Zhu, Bin
dc.contributor.author
Hernandez, Alfredo
dc.contributor.author
Tan, Min
dc.contributor.author
Wollenhaupt, Jan
dc.contributor.author
Tabor, Stanley
dc.contributor.author
Richardson, Charles C.
dc.date.accessioned
2018-06-08T03:45:00Z
dc.date.available
2015-09-25T11:16:25.664Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15855
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-20042
dc.description.abstract
The substitution of 2′-fluoro for 2′-hydroxyl moieties in RNA substantially
improves the stability of RNA. RNA stability is a major issue in RNA research
and applications involving RNA. We report that the RNA polymerase from the
marine cyanophage Syn5 has an intrinsic low discrimination against the
incorporation of 2′-fluoro dNMPs during transcription elongation. The presence
of both magnesium and manganese ions at high concentrations further reduce
this discrimination without decreasing the efficiency of incorporation. We
have constructed a Syn5 RNA polymerase in which tyrosine 564 is replaced with
phenylalanine (Y564F) that further decreases the discrimination against 2
′-fluoro-dNTPs during RNA synthesis. Sequence elements in DNA templates that
affect the yield of RNA and incorporation of 2′-fluoro-dNMPs by Syn5 RNA
polymerase have been identified.
de
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie
dc.title
Synthesis of 2′-Fluoro RNA by Syn5 RNA polymerase
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Nucleic Acids Research. - 43 (2015), 14, S. e94
dcterms.bibliographicCitation.doi
10.1093/nar/gkv367
dcterms.bibliographicCitation.url
http://nar.oxfordjournals.org/content/43/14/e94
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000023176
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005448
dcterms.accessRights.openaire
open access