dc.contributor.author
Tischer, Karsten B.
dc.contributor.author
Kaufer, Benedikt B.
dc.date.accessioned
2018-06-08T03:42:13Z
dc.date.available
2014-03-11T09:01:57.308Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15759
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19946
dc.description.abstract
Maintenance and manipulation of large DNA and RNA virus genomes had presented
an obstacle for virological research. BAC vectors provided a solution to both
problems as they can harbor large DNA sequences and can efficiently be
modified using well-established mutagenesis techniques in Escherichia coli.
Numerous DNA virus genomes of herpesvirus and pox virus were cloned into
mini-F vectors. In addition, several reverse genetic systems for RNA viruses
such as members of Coronaviridae and Flaviviridae could be established based
on BAC constructs. Transfection into susceptible eukaryotic cells of virus DNA
cloned as a BAC allows reconstitution of recombinant viruses. In this paper,
we provide an overview on the strategies that can be used for the generation
of virus BAC vectors and also on systems that are currently available for
various virus species. Furthermore, we address common mutagenesis techniques
that allow modification of BACs from single-nucleotide substitutions to
deletion of viral genes or insertion of foreign sequences. Finally, we review
the reconstitution of viruses from BAC vectors and the removal of the
bacterial sequences from the virus genome during this process.
en
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
Viral Bacterial Artificial Chromosomes
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Journal of Biomedicine and Biotechnology. - 2012 (2012), Artikel Nr. 472537
(14 S.)
dc.identifier.sepid
29332
dc.title.subtitle
Generation, Mutagenesis, and Removal of Mini-F Sequences
dcterms.bibliographicCitation.doi
10.1155/2012/472537
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1155/2012/472537
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Virologie
refubium.mycore.fudocsId
FUDOCS_document_000000019822
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000003187
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1110-7243