dc.contributor.author
Ganesh, Bhanu Priya
dc.contributor.author
Klopfleisch, Robert
dc.contributor.author
Loh, Gunnar
dc.contributor.author
Blaut, Michael
dc.date.accessioned
2018-06-08T03:38:11Z
dc.date.available
2014-01-26T18:24:56.494Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15602
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19790
dc.description.abstract
Excessive mucin degradation by intestinal bacteria may contribute to
inflammatory bowel diseases because access of luminal antigens to the
intestinal immune system is facilitated. This study investigated how the
presence of a mucin degrading commensal bacterium affects the severity of an
intestinal Salmonella enterica Typhimurium-induced gut inflammation. Using a
gnotobiotic C3H mouse model with a background microbiota of eight bacterial
species (SIHUMI) the impact of the mucin-degrading commensal bacterium
Akkermansia muciniphila (SIHUMI-A) on inflammatory and infectious symptoms
caused by S. Typhimurium was investigated. Presence of A. muciniphila in S.
Typhimurium-infected SIHUMI mice caused significantly increased histopathology
scores and elevated mRNA levels of IFN-γ, IP-10, TNF-α, IL-12, IL-17 and IL-6
in cecal and colonic tissue. The increase in pro-inflammatory cytokines was
accompanied by 10-fold higher S. Typhimurium cell numbers in mesenteric lymph
nodes of SIHUMI mice associated with A. muciniphila and S. Typhimurium
(SIHUMI-AS) compared to SIHUMI mice with S. Typhimurium only (SIHUMI-S). The
number of mucin filled goblet cells was 2- to 3-fold lower in cecal tissue of
SIHUMI-AS mice compared to SIHUMI-S, SIHUMI-A or SIHUMI mice. Reduced goblet
cell numbers significantly correlated with increased IFN-γ mRNA levels (r(2) =
-0.86, ***P<0.001) in all infected mice. In addition, loss of cecal mucin
sulphation was observed in SIHUMI mice containing both A. muciniphila and S.
Typhimurium compared to other mouse groups. Concomitant presence of A.
muciniphila and S. Typhimurium resulted in a drastic change in microbiota
composition of SIHUMI mice: the proportion of B. thetaiotaomicron in SIHUMI-AS
mice was 0.02% of total bacteria compared to 78%-88% in the other mouse groups
and the proportion of S. Typhimurium was 94% in SIHUMI-AS mice but only 2.2%
in the SIHUMI-S mice. These results indicate that A. muciniphila exacerbates
S. Typhimurium-induced intestinal inflammation by its ability to disturb host
mucus homeostasis.
de
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/deed.de
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Commensal Akkermansia muciniphila exacerbates gut inflammation in Salmonella
Typhimurium-infected gnotobiotic mice
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS one; 2013 8(9) , e74
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0074963
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1371/journal.pone.0074963
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Tierpathologie
refubium.funding
Deutsche Forschungsgemeinschaft (DFG)
refubium.mycore.fudocsId
FUDOCS_document_000000019490
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000002958
dcterms.accessRights.openaire
open access