dc.contributor.author
Kirschner, Felicia
dc.contributor.author
Reppe, Katrin
dc.contributor.author
Andresen, Nadine
dc.contributor.author
Witzenrath, Martin
dc.contributor.author
Ebstein, Frédéric
dc.contributor.author
Kloetzel, Peter-Michael
dc.date.accessioned
2018-06-08T03:33:15Z
dc.date.available
2016-05-30T10:34:21.044Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15430
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19618
dc.description.abstract
Immunoproteasomes, harboring the active site subunits β5i/LMP7, β1i/LMP2, and
β2i/MECL1 exert protective, regulatory or modulating functions during
infection-induced immune responses. Immunoproteasomes are constitutively
expressed in hematopoietic derived cells, constituting the first line of
defense against invading pathogens. To clarify the impact of immunoproteasomes
on the innate immune response against Streptococcus pneumoniae, we
characterized the progression of disease and analyzed the systemic immune
response in β5i/LMP7-/- mice. Our data show that β5i/LMP7 deficiency, which
affected the subunit composition of proteasomes in murine macrophages and
liver, was accompanied by reduced transcription of genes encoding immune
modulating molecules such as pentraxins, ficolins, and collectins. The
diminished opsonin expression suggested an impaired humoral immune response
against invading pneumococci resulting in an aggravated systemic dissemination
of S. pneumoniae in β5i/LMP7-/- mice. The impaired bacterial elimination in
β5i/LMP7-/- mice was accompanied by an aggravated course of pneumonia with
early mortality as a consequence of critical illness during the late phase of
disease. In summary our results highlight an unsuspected role for immuno-
subunits in modulating the innate immune response to extracellular bacterial
infections.
en
dc.format.extent
22 Seiten
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::572 Biochemie
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Proteasome β5i Subunit Deficiency Affects Opsonin Synthesis and Aggravates
Pneumococcal Pneumonia
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 11 (2016), 4, Artikel Nr. e0153847
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0153847
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1371/journal.pone.0153847
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000024635
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000006480
dcterms.accessRights.openaire
open access