dc.contributor.author
Malhi, M.
dc.contributor.author
Gui, H.
dc.contributor.author
Yao, L.
dc.contributor.author
Aschenbach, Jörg R.
dc.contributor.author
Gäbel, G.
dc.contributor.author
Shen, Z.
dc.date.accessioned
2018-06-08T03:32:14Z
dc.date.available
2015-06-10T10:45:59.039Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15392
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19580
dc.description.abstract
We tested the hypothesis that the proliferative effects of intraruminal
butyrate infusions on the ruminal epithelium are linked to upregulation in
cyclin D1 (CCND1), the cyclin-dependent kinase 4 (CDK4), and their possible
association with enhanced absorption of short-chain fatty acids (SCFA). Goats
(n=23) in 2 experiments (Exp.) were fed 200 g/d concentrate and hay ad
libitum. In Exp. 1, goats received an intraruminal infusion of sodium butyrate
at 0.3 (group B, n=8) or 0 (group C, n=7) g/kg of body weight (BW) per day
before morning feeding for 28 d and were slaughtered 8 h after the butyrate
infusion. In Exp. 2, goats (n=8) received butyrate infusion and feeding as in
Exp. 1. On d 28, epithelial samples were biopsied from the antrium ruminis at
0, 3, and 7 h after the last butyrate infusion. In Exp. 1, the ruminal molar
proportional concentration of butyrate increased in group B by about 110%
after butyrate infusion and remained elevated for 1.5 h; thereafter, it
gradually returned to the baseline (preinfusion) level. In group C, the molar
proportional concentration of butyrate was unchanged over the time points. The
length and width of papillae increased in B compared with C; this was
associated with increased numbers of cells and cell layers in the epithelial
strata and an increase in the surface area of 82%. The mRNA expression of
CCND1 increased transiently at 3 h but returned to the preinfusion level at 7
h following butyrate infusion in Exp. 2. However, it did not differ between B
and C in Exp. 1, in which the ruminal epithelium was sampled at 8 h after
butyrate infusion. The mRNA expression of the monocarboxylate transporter
MCT4, but not MCT1, was stably upregulated in B compared with C. The estimated
absorption rate of total SCFA (%/h) increased in B compared with C. We
conclude that transient increases in cyclin D1 transcription contribute to
butyrate-induced papillae growth and subsequently to the increased absorption
of SCFA in the ruminal epithelium of goats.
en
dc.rights.uri
http://jds.fass.org/misc/ifora.shtml
dc.subject
rumen papillae growth and short-chain fatty acid absorption
dc.subject
cyclin D1 and monocarboxylate transporters
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
Increased papillae growth and enhanced short-chain fatty acid absorption in
the rumen of goats are associated with transient increases in cyclin D1
expression after ruminal butyrate infusion
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Journal of Dairy Science. - 96 (2013), 12, S. 7603-7616
dc.identifier.sepid
37159
dcterms.bibliographicCitation.doi
10.3168/jds.2013-6700
dcterms.bibliographicCitation.url
http://dx.doi.org/10.3168/jds.2013-6700
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Veterinär-Physiologie
refubium.mycore.fudocsId
FUDOCS_document_000000022589
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005026
dcterms.accessRights.dnb
free
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
00220302