dc.contributor.author
Starke, Ingo C.
dc.contributor.author
Zentek, Jürgen
dc.contributor.author
Vahjen, Wilfried
dc.date.accessioned
2018-06-08T03:29:29Z
dc.date.available
2014-01-22T10:52:00.117Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15291
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19479
dc.description.abstract
Piglets were fed diets containing 57 (low) or 2425 (high) mg zinc from
analytical grade zinc oxide (ZnO) ·kg(-1) feed. Digesta samples from the
stomach and jejuna of 32, 39, 46 and 53 d old animals (n = 6 per group) were
incubated in media containing 80, 40, 20 and 0 µg·mL(-1) soluble zinc from
ZnO. Turbidity was recorded for 16 h and growth parameters were calculated.
Additionally, DNA extracts of selected samples were analyzed via qPCR for
different bacterial groups. Samples from animals fed the low dietary zinc
concentration always showed highest rate of growth and lowest lag times in
media without added zinc. However, media supplemented with zinc displayed
highest growth rates and lowest lag time in the high dietary zinc group.
Specific growth rates and lag time showed significant differences on day 32
and 39 of age, but rarely on days 46 and 53 of age. Bacterial growth in
digesta samples from the high dietary zinc group was less influenced by zinc
and recovered growth more rapidly than in the low dietary zinc group. Specific
growth rates and bacterial cell numbers from qPCR results showed that
lactobacilli were most susceptible to zinc, while bifidobacteria,
enterobacteria and enterococci exhibited increased growth rates in samples of
animals from the high dietary zinc treatment. No treatment related differences
were observed for clostridial cluster IV and the Bacteroides-Prevotella-
Porphyromonas cluster. The diversity of enterobacteria after incubation was
always higher in the high dietary zinc treatment or in medium supplemented
with 80 µg·mL(-1) soluble ZnO. This study has shown that a pharmacological
dosage of ZnO leads to a reduced ex vivo-bacterial growth rate of bacteria
from the stomach and jejunum of weaned piglets. In view of the rapid bacterial
adaptation to dietary zinc, the administration of ZnO in feeds for weaned
piglets might only be beneficial in a short period after weaning.
en
dc.rights.uri
http://www.creativecommons.org/licenses/by-nc/3.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft
dc.title
Ex vivo-growth response of porcine small intestinal bacterial communities to
pharmacological doses of dietary zinc oxide
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS one; 8(2): e56405
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0056405
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1371/journal.pone.0056405
refubium.affiliation
Veterinärmedizin
de
refubium.affiliation.other
Institut für Tierernährung

refubium.mycore.fudocsId
FUDOCS_document_000000019461
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000002941
dcterms.accessRights.openaire
open access