dc.contributor.author
Pafumi, Irene
dc.contributor.author
Favia, Annarita
dc.contributor.author
Gambara, Guido
dc.contributor.author
Papacci, Francesca
dc.contributor.author
Ziparo, Elio
dc.contributor.author
Palombi, Fioretta
dc.contributor.author
Filippini, Antonio
dc.date.accessioned
2018-06-08T03:27:33Z
dc.date.available
2015-07-17T06:56:08.076Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15216
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19404
dc.description.abstract
Angiopoietins are vascular factors essential for blood vessel assembly and
correct organization and maturation. This study describes a novel calcium-
dependent machinery activated through Angiopoietin-1/2-Tie receptor system in
HUVECs monolayer. Both cytokines were found to elicit intracellular calcium
mobilization. Targeting intracellular Ca2+ signaling, antagonizing IP3 with
2-APB or cADPR with 8Br-cADPR, was found to modulate in vitro angiogenic
responses to Angiopoietins in a specific way. 2-APB and 8Br-cADPR impaired the
phosphorylation of AKT and FAK induced by Ang-1 and Ang-2. On the other hand,
phosphorylation of ERK1/2 and p38, as well as cell proliferation, was not
affected by either inhibitor. The ability of ECs to migrate following Angs
stimulation, evaluated by “scratch assay,” was reduced by either 2-APB or 8Br-
cADPR following Ang-2 stimulation and only slightly affected by 2-APB in cells
stimulated with Ang-1. These results identify a novel calcium-dependent
machinery involved in the complex interplay regulating angiogenic processes
showing that IP3- and cADPR-induced Ca2+ release specifically regulates
distinct Angs-mediated angiogenic steps.
de
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Regulation of Angiogenic Functions by Angiopoietins through Calcium-Dependent
Signaling Pathways
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
BioMed Research International. - 2015 (2015), Artikel Nr. 965271
dcterms.bibliographicCitation.doi
10.1155/2015/965271
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1155/2015/965271
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000022843
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000005220
dcterms.accessRights.openaire
open access